THE mature vesicle of Valonia ventricosa was an early source of information about the nature of cellulose1 and the configuration of a cellulose cell wall2,3. Later it was used in one of the first investigations with the electron microscope of the way a plant cell wall develops at a naked protoplasmic surface4. The opportunity to make such a study was provided by the formation, at will, of aplanospores and by their subsequent development5. Aplanospores are small spherical masses of protoplasm, each with a nucleus, and they may form in very large numbers from the multinucleate, non-septate, protoplasm within a single vesicle. This system, therefore, has also provided an opportunity to allow sporelings to develop their cell walls while in contact with 14C-proline in sea-water. Current methods of autoradiography and electron microscopy make possible determination of the distribution of the radioactivity fixed from 14C-proline. This should test whether a developing cellulose cell wall incorporates substantial amounts of “cell wall protein” as indicated by radioactivity bound in the form of 14C-proline. Also the cell wall of mature vesicles of V. ventricosa can easily be obtained free of cytoplasm so that it is possible to ascertain, by direct analysis, how much total protein each contains and its content of proline and/or hydroxyproline.
Subscribe to Journal
Get full journal access for 1 year
only $3.83 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Sponsler, O. L., Protoplasma, 12, 241 (1931).
Astbury, W. T., Marwick, T. C., and Bernal, J. D., Proc. Roy. Soc., B, 109, 443 (1932).
Preston, B. D., and Astbury, W. T., Proc. Roy. Soc., B, 122, 76 (1937).
Steward, F. C., and Mühlethaler, K., Ann. Bot., N.S., 17, 295 (1953).
Steward, F. C., Papers from Tortugas Lab., 32, Carnegie Inst. Washington, Publ. No. 517, 87 (1939).
Lamport, D. T. A., Adv. Bot. Res., 2, 151 (1965).
Thompson, E. W., and Preston, R. D., Nature, 213, 684 (1967).
Gotelli, I. B., and Cleland, R., Amer. J. Bot., 55, 907 (1968).
Thompson, E. W., and Preston, R. D., J. Exp. Bot., 19, 690 (1968).
Punnet, T., and Derrenbacker, E. C., J. Gen. Microbiol., 44, 105 (1966).
Israel, H. W., Salpeter, M. M., and Steward, F. C., J. Cell Biol., 39, 698 (1968).
Nature, 221, 123 (1969).
Steward, F. C., Thompson, J. F., and Pollard, J. K., J. Exp. Bot., 9, 1 (1958).
Pollard, J. K., and Steward, F. C., J. Exp. Bot., 10, 17 (1959).
Steward, F. C., Kent, A. E., and Mapes, M. O., in Current Topics in Developmental Biology (edit. by Monroy, M. A., and Moscona, A. A.), 1, 113 (Academic Press, New York, 1966).
Steward, F. C., and Mott, R. L., Intern. Rev. Cytol., 28 275 (1970).
Lyndon, R. F., and Steward, F. C., J. Exp. Bot., 14, 42 (1963).
Doyle, W. L., Papers from Tortugas Lab., 29, Carnegie Inst. Washington, Publ. No. 452, 13 (1935).
Cronshaw, J., Myers, A., and Preston, R. D., Biochim. Biophys. Acta, 27. 89 (1958).
Cleland, R., Plant Physiol., 42, 669 (1967).
Cleland, R., Plant Physiol., 43, 865 (1968).
Savada, D., and Chrispeels, M. J., Science, 165, 299 (1969).
Knox, R. B., and Heslop-Harrison, J., Nature, 223, 92 (1969).
Lamport, D. T. A., Biochemistry, 8, 1155 (1969).
About this article
Cite this article
STEWARD, F., MOTT, R., ISRAEL, H. et al. Proline in the Vesicles and Sporelings of Valonia ventricosa and the Concept of Cell Wall Protein. Nature 225, 760–763 (1970). https://doi.org/10.1038/225760a0
Fractionation and characterisation ofj water-soluble polysaccharide-protein complexes containing hydroxyproline from the leaves ofVida faba
Journal of the Science of Food and Agriculture (1971)
Journal of Phytopathology (1971)