Abstract
MUCH of the protein detectable in short-term leachates from intact pollen grains1 is probably released from the inner layer of the wall, the cellulosic intine, where protein and enzyme activity have been detected by cytochemical methods2,3. Extracts from intact grains are usually used in tests for pollen allergenicity, and we have considered the possibility that the proteins principally responsible come from the wall sites and not from, within the cells. We report here the results of a study of the antigens present in leachates in which we have shown by immunofluorescence that they are indeed contributed largely from the intine and other wall locations.
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References
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KNOX, R., HESLOP-HARRISON, J. & REED, C. Localization of Antigens associated with the Pollen Grain Wall by Immunofluorescence. Nature 225, 1066–1068 (1970). https://doi.org/10.1038/2251066a0
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DOI: https://doi.org/10.1038/2251066a0
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