Abstract
A GROUP of hereditary non-spherocytic anaemias have been shown to be associated with unstable haemoglobins through the application of a simple screening test for thermolability involving incubation of haemolysate at 50° C in phosphate buffer (pH 6.5–7.4; 0.1 M)1–3. Unstable haemoglobins form a precipitate within a few hours but normal haemoglobin remains in solution. We have encountered two families with this type of anaemia whose haemoglobins demonstrated little or no precipitate in these conditions, but formed copious precipitate in the presence of non-phosphate buffers—observations which led us to wonder whether the recently described effects of phosphates on the oxygen-binding properties of haemoglobin4–7 might not also modify the heat stability of the molecule.
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SCHNEIDERMAN, L., JUNGA, I. & FAWLEY, D. Effect of Phosphate and Non-phosphate Buffers on Thermolability of Unstable Haemoglobins. Nature 225, 1041–1042 (1970). https://doi.org/10.1038/2251041a0
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DOI: https://doi.org/10.1038/2251041a0
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