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Isolation of Pure lac Operon DNA*

Abstract

Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription.

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SHAPIRO, J., MACHATTIE, L., ERON, L. et al. Isolation of Pure lac Operon DNA*. Nature 224, 768–774 (1969). https://doi.org/10.1038/224768a0

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  • DOI: https://doi.org/10.1038/224768a0

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