Crystals of Fragment Fab′: Preparation from Pepsin Digests of Human IgG Myeloma Proteins


INFORMATION concerning the three-dimensional structure of an antibody and the contours of its combining site can in theory be derived by X-ray crystallography. In view of the large molecular weight of antibody molecules (150,000 or greater), crystals of fragment Fab, which has a molecular weight of 50,000 and contains the combining site of an antibody, may be of particular value.

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  1. 1

    Rossi, G., and Nisonoff, A., Biochem. Biophys. Res. Commun., 31, 914 (1968).

  2. 2

    Humphrey, R. L., Avey, H. P., Becka, L. N., Poljak, R. J., Rossi, G., Choi, T. K., and Nisonoff, A., J. Mol. Biol. (in the press).

  3. 3

    Avey, H. P., Poljak, R. J., Rossi, G., and Nisonoff, A., Nature, 220, 1248 (1968).

  4. 4

    Terry, W. D., Matthews, B. W., and Davies, D. R., Nature, 220, 239 (1968).

  5. 5

    Givol, D., and DeLorenzo, F., J. Biol. Chem., 243, 1886 (1968).

  6. 6

    Steiner, L. A., and Porter, R. R., Biochemistry, 6, 3957 (1967).

  7. 7

    Gall, W. E., Cunningham, B. A., Waxdal, M. J., Konisberg, W. M., and Edelman, G. M., Biochemistry, 7, 1973 (1968).

  8. 8

    Nisonoff, A., Wissler, F. C., Lipman, L. N., and Woernley, D. L., Arch. Biochem. Biophys., 89, 230 (1960).

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ROSSI, G., CHOI, T. & NISONOFF, A. Crystals of Fragment Fab′: Preparation from Pepsin Digests of Human IgG Myeloma Proteins. Nature 223, 837–838 (1969).

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