Abstract
WE have recently prepared the protein moiety or apoprotein of beta lipoprotein (βLP) in a soluble form that is similar but not identical to the parent βLP with respect to immunochemical and optical properties1–3. The occurrence of lipid-free β-apoprotein in the plasma of normals and patients with abetalipoproteinaemia (ABL) has been inferred from experiments in which antisera to ABL plasma and to the plasma fraction of density greater than 1.21 (d 1.21 infranatant) were found to react with the acetyl and azo-derivatives of βLP4. These derivatives differed in immunochemical properties from βLP and seemed to be identical to an antigen present in the plasma of normals or patients with ABL. It was concluded that this antigen was most likely β-apoprotein and that β-apoprotein may exist in an altered antigenic and conformational state in the d 1.21 infranatant fraction. Our study attempts to make a direct comparison of the immunochemical reactivities of β-apoprotein and the chemical derivatives.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gotto, A. M., Levy, R. I., Rosenthal, A. S., and Fredrickson, D. S., Nature, 219, 1157 (1968).
Gotto, A. M., Levy, R. I., Rosenthal, A. S., Birnbaumer, M. E., and Fredrickson, D. S., Biochem. Biophys. Res. Commun., 31, 699 (1968).
Gotto, A. M., Levy, R. I., and Fredrickson, D. S., Proc. US Nat. Acad. Sci., 60, 1436 (1968).
Lees, R. S., J. Lipid Res., 8, 396 (1967).
Gotto, A. M., Levy, R. I., and Fredrickson, D. S., Biochem. Biophys. Res. Commun., 31, 151 (1968).
Hass, L. F., Biochemistry, 3, 535 (1964).
Riordan, J. F., and Vallee, B. L., Biochemistry, 3, 1768 (1964).
Tabachnick, M., and Sobotka, H. J., J. Biol. Chem., 235, 1051 (1960).
Margolis, S., and Langdon, R. G., J. Biol. Chem., 241, 477 (1966).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Bot Chem., 193, 265 (1951).
Moore, S., and Stein, W. H., J. Biol. Chem., 176, 367 (1948).
Margolis, S., and Langdon, R. G., J. Biol. Chem., 241, 469 (1966).
Wolfsy, L., and Singer, S. J., Biochemistry, 2, 104 (1963).
Levy, R. I., and Fredrickson, D. S., J. Clin. Invest., 44, 426 (1965).
Levy, R. I., Lees, R. S., and Fredrickson, D. S., J. Clin. Invest., 45, 63 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GOTTO, A., LEVY, R., BIRNBAUMER, M. et al. Human Serum Beta Lipoprotein and Beta Apoprotein. Nature 223, 835–837 (1969). https://doi.org/10.1038/223835a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/223835a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
