Uptake of Calcium by Platelet Relaxing Factor

Abstract

IN 1963 Grette1 described a relaxing factor in the 14,000g supernatant obtained after extraction of homogenized platelets in salt solution. In the presence of ATP the apparently membrane-free solution inhibited superprecipitation of the platelet contractile protein, thrombosthenin2, and the inhibitory activity was abolished by addition of excess calcium ions. Grette's findings were identical to results obtained by others on the behaviour of actomyosin in the presence of ATP and relaxing factor from skeletal muscle3. The relaxing factors extracted from muscle and platelets were thought to be soluble components of the respective cells1,3.

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STATLAND, B., HEAGAN, B. & WHITE, J. Uptake of Calcium by Platelet Relaxing Factor. Nature 223, 521–522 (1969). https://doi.org/10.1038/223521a0

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