Letter | Published:

In vitro Evaluation of Immunosuppressive Drugs

Naturevolume 222pages998999 (1969) | Download Citation

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Abstract

In vivo testing of immunosuppressive drugs is possible only in animals and the results cannot be extrapolated to humans. None of the in vitro tests used so far has proved satisfactory. The new in vitro method described here is sensitive enough for the testing of metabolites present in human serum after the administration of drugs. It is based on the inhibition of spontaneous rosette forming cells (RFC), the nature of formation of which may differ from that of immune rosette formation. This spontaneous phenomenon is usually supposed to result from natural antibody producing cells, and the number of spontaneous RFC in mice with sheep red blood cells (SRBC) is more than ten times smaller than in mice immunized with SRBC1.

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References

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    Biozzi, G., Stiffel, C., Mouton, D., Liacopoulos-Briot, M., Ducreusefond, C., and Bouthillier, Y., Ann. Inst. Pasteur, 110, 7 (1966).

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Affiliations

  1. Hôpital Necker, 151 rue de Sèvres, Paris

    • J. F. BACH
    • , M. DARDENNE
    •  & C. FOURNIER

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https://doi.org/10.1038/222998a0

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