Abstract
MITOTIC apparatuses (MA) isolated from sea urchin eggs are not functional1, and although their biochemical composition1–5, stability6 and morphological characteristics7–10 have been studied, isolated MA may be quite different from in vivo MA. In particular, MA may lose material during isolation11, and the birefringence may be altered. We therefore studied the effect of the isolation procedure on mass and birefringence.
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References
Mazia, D., in The Cell, 3 (edit. by Brachet, J., and Mirsky, A. E.) (Academic Press, New York, 1961).
Zimmerman, A. M., Exp. Cell Res., 20, 529 (1960).
Sakai, H., Biochim. Biophys. Acta, 112, 132 (1966).
Kane, R. E., J. Cell Biol., 32, 243 (1967).
Stephens, R. E., J. Cell Biol., 32, 255 (1967).
Kane, R. E., J. Cell Biol., 25 (No. 1, Pt. 2), 137 (1965).
Kane, R. E., J. Cell Biol., 15, 279 (1962).
Kane, R. E., and Forer, A., J. Cell Biol., 25 (No. 3, Pt. 2), 31 (1965).
Rebhun, L. I., and Sander, G., J. Cell Biol., 34, 859 (1967).
Goldman, R. D., and Rebhun, L. I., J. Cell Sci., 4, 179 (1969).
Forer, A., in Handbook of Molecular Cytology (edit. by Lima-de-Faria, A.) (North-Holland Publishing Co., Amsterdam, in the press, 1969).
Hale, A., The Interference Microscope in Biological Research (Livingstone, Edinburgh, 1958).
Ross, K. F. A., Phase Contrast and Interference Microscopy for Cell Biologists (Arnold, London, 1967).
Bennett, H. S., in McClung's Handbook of Microscopical Technique (edit. by McChung-Jones, R.) (Hoeber, New York, 1950).
Inoué, S., in Encyclopedia of Microscopy (edit. by Clark G.) (Reinhold, New York, 1961).
Ballentine, R., J. Cel. Comp. Physiol., 15, 121 (1940).
Krahl, M. E., Biol. Bull. Mar. Biol. Lab., Woods Hole, 98, 175 (1950).
Mitchison, J. M., and Swann, M. M., Quart. J. Microsc. Sci., 94, 381 (1953).
Borisy, G., and Taylor, E. W., J. Cell Biol., 34, 535 (1967).
Bibring, T., and Baxandal, J., Science, 161, 377 (1968).
Hartmann, J. F., and Zimmerman, A. M., Exp. Cell Res., 50, 403 (1968).
Harvey, E. B., The American Arbacia and Other Sea Urchins (Princeton Univ. Press, Princeton, 1956).
Goldman, R. D., thesis, Princeton Univ. (1967).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Warburg, O., and Christian, W., Biochem. Z., 310, 384 (1941).
Wilt, F. H., Sakai, H., and Mazia, D., J. Mol. Biol., 27, 1 (1967).
Miki-Noumara, T., Exp. Cell Res., 50, 54 (1968).
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FORER, A., GOLDMAN, R. Comparisons of Isolated and in vivo Mitotic Apparatuses. Nature 222, 689–691 (1969). https://doi.org/10.1038/222689a0
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DOI: https://doi.org/10.1038/222689a0
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