Letter | Published:

Effect of Hydrocortisone on the Keratinization of Chick Embryonic Skin cultured in a Chemically Defined Medium

Nature volume 222, pages 12701272 (28 June 1969) | Download Citation



DURING keratinization of the epidermis—a process of cell maturation—synthesis of specific protein(s) within the cells is accompanied by degradation of the cellular constituents. Hormones may be involved as controlling factors. This has been one of the most attractive topics of tissue culture studies ever since Strangeways and Fell1 cultured chick embryonic skin by the watchglass method using a natural plasma clot medium. Later, Fell et al.2,3 found that hydrocortisone accelerated the keratinization of chick and rat embryonic skin cultured on a plasma clot. Chemically defined media have been used successfully for cultivating rat4, chick5,6 and human7 foetal skin, and Wessells5 demonstrated that thyroxine initiated keratinization of the epidermis of chick embryonic skin growing in such media. These histological findings suggest that hormones are probably important in keratinization in embryonic development.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1.

    , and , Proc. Roy. Soc., B, 99, 340 (1925–26).

  2. 2.

    , J. Embryol. Exp. Morphol., 10, 389 (1962).

  3. 3.

    , and , J. Exp. Med., 116, 365 (1962).

  4. 4.

    , Exp. Cell Res., 16, 118 (1959).

  5. 5.

    , Exp. Cell Res., 24, 131 (1961).

  6. 6.

    , Develop. Biol., 3, 355 (1961).

  7. 7.

    , J. Pathol. Bact., 88, 203 (1964).

  8. 8.

    , , and , Exp. Cell Res., 25, 44 (1961).

Download references

Author information


  1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo.



  1. Search for MASANOBU SUGIMOTO in:

  2. Search for HIROYOSHI ENDO in:

About this article

Publication history






By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.