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Platelet Factor 4 and Adenosine Diphosphate Release during Human Platelet Aggregation

Naturevolume 222pages12691270 (1969) | Download Citation



PLATELET FACTOR 4 (PF4) is a low molecular weight, heat stable protein which neutralizes heparin1,2. Recent reports indicate PF4 is released from human platelets during aggregation by adenosine diphosphate (ADP), adrenaline, thrombin and collagen3,4. It is known that human platelet aggregation in plasma containing citrate proceeds in two phases in response to adrenaline, ADP and thrombin5–8. Reversible platelet aggregation seems to be chiefly the result of changes in the platelet membrane induced by external stimuli. The second phase is accompanied by the selective release of various platelet contents, including adenine nucleotides, 5-hydroxytryptamine and certain lysosomal enzymes9. We have examined the release of PF4 in human platelet-rich plasma with particular reference to the two phases of platelet aggregation. We also compared release of PF4 with ADP release.

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  1. Vascular Laboratory, Lemuel Shattuck Hospital, and Tufts University School of Medicine, Boston, Massachusetts, 02130



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