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Inhibition of CO2 Combination and Reduction of the Bohr Effect in Haemoglobin chemically modified at its α-Amino Groups

Naturevolume 222pages12431246 (1969) | Download Citation

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Abstract

Blocking of the α-amino groups by cyanate inhibits the uptake of CO2 by haemoglobin. It also inhibits the influence which changes in pCO2 at constant pH normally have on the oxygen affinity and on the Bohr effect. Blocking the α-amino groups of the α chain reduces the alkaline Bohr effect by 25 per cent.

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References

  1. 1

    Ferguson, J. K. W., and Roughton, F. J. W., J. Physiol., 83, 68 (1934).

  2. 2

    Ferguson, J. K. W., and Roughton, F. J. W., J. Physiol., 83, 87 (1934).

  3. 3

    Ferguson, J. K. W., J. Physiol., 88, 40 (1936).

  4. 4

    Rossi-Bernardi, L., and Roughton, F. J. W., J. Physiol., 189, 1 (1967).

  5. 5

    Faurolt, C., J. Chim. Phys., 22, 1 (1925). Siegfried, H., Z. Physiol. Chem., 44, 85 (1905).

  6. 6

    Tanford, C., Physical Chemistry of Macromolecules (Wiley, New York, 1962).

  7. 7

    Tanford, C., and Nozaki, Y., J. Biol. Chem., 241, 2832 (1966).

  8. 8

    Rossi-Bernardi, L., and Roughton, F. J. W., J. Biol. Chem., 242, 784 (1967).

  9. 9

    Hill, R. J., and Davis, R. W., J. Biol. Chem., 242, 2005 (1967).

  10. 10

    Stark, G. R., and Smyth, D. G., J. Biol. Chem., 238, 214 (1963).

  11. 11

    Stark, G. R., Biochemistry, 4, 1030 (1965).

  12. 12

    Smyth, D. G., J. Biol. Chem., 242, 1579 (1967).

  13. 13

    Perutz, M. F., Muirhead, H., Cox, J. M., and Goaman, L. C. G., Nature, 219, 131 (1968).

  14. 14

    Bolton, W., Cox, J. M., and Perutz, M. F., J. Mol. Biol., 33, 283 (1968).

  15. 15

    Bangham, A. D., and Lehmann, H., Nature, 181, 267 (1958).

  16. 16

    Kilmartin, J. V., and Clegg, J. B., Nature, 213, 269 (1967).

  17. 17

    Clegg, M. D., and Schroeder, W. A., J. Amer. Chem. Soc., 81, 6065 (1959).

  18. 18

    Taylor, J. F., Antonini, E., Brunori, M., and Wyman, J., J. Biol. Chem., 241, 241 (1966).

  19. 19

    Clegg, J. B., Naughton, M. A., and Weatherall, D. J., J. Mol. Biol., 19, 91 (1966).

  20. 20

    Kilmartin, J. V., and Rossi-Bernardi, L., Symposium on CO2 (International Union of Physiological Sciences, 1968, in the press).

  21. 21

    Riggs, A. F., J. Gen. Physiol., 35, 23 (1951).

  22. 22

    Margaria, R., and Green, A. A., J. Biol. Chem., 102, 611 (1933).

  23. 23

    Kernohan, J. C., Kreuzer, F., Rossi-Bernardi, L., and Roughton, F. J. W., Biochem. J., 100, 49P (1966).

  24. 24

    Stadie, W. C., and O'Brien, H., J. Biol. Chem., 117, 439 (1937).

  25. 25

    Perutz, M. F., Muirhead, H., Mazzarella, L., Crowther, R. G. A., Greer, J., and Kilmartin, J. V., preceding paper, Nature, 222, 1240 (1969).

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Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge

    • J. V. KILMARTIN
  2. Istituto di Chimica Organica, University of Milan, Milan

    • L. ROSSI-BERNARDI

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https://doi.org/10.1038/2221243a0

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