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Aconitase, a Complex with Two Active Centres

Nature volume 221, pages 774–775 (1969)Cite this article

Abstract

WE have found that the reactions citrate ⇌ cis-aconitate (i) isocitrate ⇌ cis aconitate (ii) can be separated during a controlled ageing at 0° C of aconitase (aconitate hydratase, E.C. 4.2.1.3.), purified as far as the first ethanol stage in Morrison's preparation from pig heart. At this stage, the enzyme still contains the Fe2+ combined with the enzyme, so that no activation is necessary1. In Fig. 1, curves (a) and (b) give the initial rates for (ii) and (i) respectively, ratio (i)/(ii) 1.00. After 6 days at 0° C, the rates observed in the same conditions were (c) and (d), making the ratio 0.18.

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Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Cambridge

    R. A. PETERS & M. SHORTHOUSE

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  1. R. A. PETERS
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  2. M. SHORTHOUSE
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PETERS, R., SHORTHOUSE, M. Aconitase, a Complex with Two Active Centres. Nature 221, 774–775 (1969). https://doi.org/10.1038/221774a0

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