Abstract
MOLECULAR hybridizations between DNA from normal mice and RNAs from immunocompetent cells exposed to antigen showed that (a) new species of RNA appeared in cells stimulated by antigen1, and (b) different species of RNA appeared in immunocompetent cells exposed to different antigens2. Similar results were obtained by Church et al.3. In the work reported here, RNAs from immunocompetent cells exposed to sheep red blood cells (RBC) were hybridized with DNA immobilized in a nitrocellulose column. The RNAs which failed to form hybrids and passed through the column were analysed.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cohen, E. P., Proc. US Nat. Acad. Sci., 57, 673 (1967).
Raska, jun., K., and Cohen, E. P., Nature, 217, 720 (1968).
Church, R. B., Storb, U., McCarthy, B. J., and Weiser, R. S., J. Immunol., 107, 399 (1968).
Marmur:, J., J. Mol. Biol., 3, 208 (1961).
Cohen, E. P., Newcomb, R. W., and Crosby, L. K., J. Immunol., 95, 583 (1965).
Bautz, E. K. F., and Reilly, E., Science, 151, 328 (1966).
Mosier, D. E., and Cohen, E. P., Nature, 219, 969 (1968).
Thor, D. E., Science, 157, 1569 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
COHEN, E. Partial Characterization by Molecular Hybridization of RNAs from Immunocompetent Cells exposed to Antigen. Nature 221, 685–687 (1969). https://doi.org/10.1038/221685a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/221685a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
