Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Structure of Subtilisin BPN′ at 2.5 Å Resolution

Nature volume 221, pages 235–242 (1969)Cite this article

Abstract

The single polypeptide chain is folded into three parts, with the active site at their conjunction. In the active enzyme, the side chain of His 64 is in a position consistent with the formation of a hydrogen bond between it and the OH group of Ser 221.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Welker, N. E., and Campbell, L. L., J. Bact., 94, 1124 (1967).

    CAS  PubMed  Google Scholar 

  2. Drenth, J., and Hol, W. G. J., J. Mol. Biol., 28, 543 (1967).

    Article  CAS  Google Scholar 

  3. Hunt, J. A., and Ottesen, M., Biochim. Biophys. Acta, 48, 411 (1961).

    Article  CAS  Google Scholar 

  4. Johansen, G., and Ottesen, M., CR Lab. Carlsberg, Sér. Chim., 34, 199 (1964).

    CAS  Google Scholar 

  5. Smith, E. L., Abstr. Seventh Intern. Cong. Biochem., Tokyo, I-1, 5, p. 9 (1967).

    Google Scholar 

  6. Smith, E. L., Markland, F. S., Kasper, C. B., DeLange, R. J., Landon, M., and Evans, W. H., J. Biol. Chem., 241, 5974 (1966).

    CAS  PubMed  Google Scholar 

  7. Gounaris, A., and Ottesen, M., CR Lab. Carlsberg, Sér. Chim., 35, 37 (1965).

    CAS  Google Scholar 

  8. Markland, F. S., and Smith, E. L., J. Biol. Chem., 242, 5198 (1967).

    CAS  PubMed  Google Scholar 

  9. Smith, E. L., DeLange, R. J., Evans, W. H., Landon, M., and Markland, F. S., J. Biol. Chem., 243, 2184 (1968).

    CAS  PubMed  Google Scholar 

  10. Markland, F. S., Ribadeau-Dumas, B., and Smith, E. L., J. Biol. Chem., 242, 5174 (1967).

    CAS  PubMed  Google Scholar 

  11. Barel, A. O., and Glazer, A. N., J. Biol. Chem., 243, 1344 (1968).

    CAS  PubMed  Google Scholar 

  12. Johansen, J. T., Ottesen, M., and Svendsen, I., Biochim. Biophys. Acta, 139, 211 (1967).

    Article  CAS  Google Scholar 

  13. Ottesen, M., and Wollenberger, A., Nature, 170, 801 (1952).

    Article  ADS  CAS  Google Scholar 

  14. Ottesen, M., and Spector, A., CR Lab. Carlsberg, Sér. Chim., 32, 63 (1960).

    CAS  Google Scholar 

  15. Tuppy, H., Monats. Chem., 84, 996 (1953).

    Article  CAS  Google Scholar 

  16. Ottesen, M., Johansen, J., Svendsen, I., and Wybrandt, G., Abstr. Seventh Intern. Cong. Biochem., Tokyo, F-211, 801 (1967).

    Google Scholar 

  17. Richards, F. M., and Vithayathil, P. J., J. Biol. Chem., 234, 1459 (1959).

    CAS  PubMed  Google Scholar 

  18. Gordillo, G., Vithayathil, P. J., and Richards, F. M., Yale J. Biol. and Med., 34, 582 (1962).

    CAS  Google Scholar 

  19. Doscher, M. S., and Hirs, C. H. W., Biochemistry, 6, 304 (1967).

    Article  CAS  Google Scholar 

  20. Gross, E., and Witkop, B., Biochemistry, 6, 745 (1967).

    Article  CAS  Google Scholar 

  21. Polgar, L., and Bender, M. L., Biochemistry, 6, 610 (1967).

    Article  CAS  Google Scholar 

  22. Neet, K. E., and Koshland, D. E., Proc. US Nat. Acad. Sci., 56, 1606 (1966).

    Article  ADS  CAS  Google Scholar 

  23. Farney, D. E., and Gold, A. M., J. Amer. Chem. Soc., 85, 997 (1963).

    Article  Google Scholar 

  24. Ottesen, M., and Schellmann, C. G., CR Lab. Carlsberg, Sér. Chim., 30, 157 (1957).

    CAS  Google Scholar 

  25. Matsubara, H., and Nishimura, S., J. Biochem., 45, 503 (1958).

    Article  Google Scholar 

  26. Jansen, E. F., Nutting, D., Jang, R., and Balls, A. K., J. Biol. Chem., 179, 189 (1949).

    CAS  PubMed  Google Scholar 

  27. Schonbaum, G. R., Zerner, B., and Bender, M. L., J. Biol. Chem., 236, 2930 (1961).

    CAS  PubMed  Google Scholar 

  28. Bernhard, S. A., Lau, S. J., and Noller, H., Biochemistry, 4, 1108 (1965).

    Article  CAS  Google Scholar 

  29. Keizer, J., and Bernhard, S. A., Biochemistry, 5, 4127 (1966).

    Article  CAS  Google Scholar 

  30. Zerner, B., and Bender, M. L., J. Amer. Chem. Soc., 86, 3669 (1964).

    Article  CAS  Google Scholar 

  31. Kaufmann, S., Schwert, G. W., and Neurath, H., Arch. Biochem. Biophys., 17, 203 (1948).

    Google Scholar 

  32. Glazer, A. N., J. Biol. Chem., 242, 433 (1967).

    CAS  PubMed  Google Scholar 

  33. Green, N. M., and Neurath, H., The Proteins, 2, 1057 (Academic Press, New York, 1954).

    Book  Google Scholar 

  34. Schoellmann, G., and Shaw, E., Biochemistry, 2, 252 (1963).

    Article  CAS  Google Scholar 

  35. Shaw, E., Mares-Guia, M., and Cohen, W., Biochemistry, 4, 2219 (1965).

    Article  CAS  Google Scholar 

  36. Sigler, P. B., Blow, D. M., Mathews, B. W., and Henderson, R., J. Mol. Biol., 35, 143 (1968).

    Article  CAS  Google Scholar 

  37. Svendsen, I., CR Lab. Carlsberg, Sér. Chim., 36, 235 (1967).

    CAS  Google Scholar 

  38. Hartley, B., Nature, 201, 1284 (1964).

    Article  ADS  CAS  Google Scholar 

  39. Walsh, K. A., and Neurath, H., Proc. US Nat. Acad. Sci., 52, 884 (1964).

    Article  ADS  CAS  Google Scholar 

  40. Structure and Activity of Enzymes (edit. by Goodwin, T. W., Harris, J. I., and Hartley, B. S.) (Academic Press, London, 1964).

  41. Sanger, F., and Shaw, D. C., Nature, 187, 872 (1960).

    Article  ADS  CAS  Google Scholar 

  42. Wang, J. H., Science, 161, 328 (1968).

    Article  ADS  CAS  Google Scholar 

  43. Wyckoff, H. W., Doscher, M., Tsernoglou, D., Inagami, T., Johnson, L. N., Hardman, K. D., Allewell, N. M., Kelly, D. M., and Richards, F. M., J. Mol. Biol., 27, 563 (1967).

    Article  CAS  Google Scholar 

  44. Furnas, T. C., Single Crystal Orienter Instruction Manual (General Electric Company, Milwaukee, 1967).

    Google Scholar 

  45. Wright, C. G., thesis, University Calif., San Diego (1969).

  46. Blow, D. M., and Crick, F. H. C., Acta Cryst., 12, 794 (1959).

    Article  CAS  Google Scholar 

  47. Kraut, J., J. Mol. Biol., 35, 511 (1968).

    Google Scholar 

  48. Weber, B. H., and Kraut, J., Biophys. Biochem. Res. Commun., 33, 280 (1968).

    Article  CAS  Google Scholar 

  49. Némethy, G., Phillips, D. C., Leach, S. J., and Scheraga, H. A., Nature, 214, 363 (1967).

    Article  ADS  Google Scholar 

  50. Pauling, L., and Corey, R. B., Proc. US Nat. Acad. Sci., 39, 253 (1953).

    Article  ADS  CAS  Google Scholar 

  51. Johnson, C. K., A Fortran Thermal-Ellipsoid Plot Program ORNL-3794 (Oak Ridge National Laboratory, Tennessee, 1965).

    Google Scholar 

  52. Wyckoff, H. W., Hardman, K. D., Allewell, N. M., Inagami, T., Johnson, L. N., and Richards, F. M., J. Biol. Chem., 242, 3984 (1967).

    CAS  PubMed  Google Scholar 

  53. Kretsinger, R. H., J. Mol. Biol., 31, 315 (1968).

    Article  CAS  Google Scholar 

  54. Perutz, M. F., Kendrew, J. C., and Watson, H. C., J. Mol. Biol., 13, 669 (1965).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, University of California, San Diego, La Jolla, California

    CHRISTINE SCHUBERT WRIGHT, RICHARD A. ALDEN & JOSEPH KRAUT

Authors
  1. CHRISTINE SCHUBERT WRIGHT
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. RICHARD A. ALDEN
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. JOSEPH KRAUT
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

WRIGHT, C., ALDEN, R. & KRAUT, J. Structure of Subtilisin BPN′ at 2.5 Å Resolution. Nature 221, 235–242 (1969). https://doi.org/10.1038/221235a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/221235a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing