Abstract
IT has been known for many years that quantitative as well as qualitative information can be derived from the Edman sequential degradation method1,2, but the variable recovery of liberated thiohydantoin derivatives of the constituent amino-acids has made an accurate quantitative analysis of proteins and pep tides difficult. We report here an approach to a quantitative protein degradation method using a volatile Edman reagent (methyl isothiocyanate3), an isotope dilution step for quantitation of the data, and an isotope ratio assay using conventional mass spectrometry4.
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References
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Waterfield, M. D., Lovins, R. E., Richards, Frank F., Salomone, R., Smith, G. P., and Haber, E., Fed. Proc., 27, 1341 (1968).
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RICHARDS, F., BARNES, W., LOVINS, R. et al. Quantitative Approach to the Sequential Degradation of Proteins and Peptides. Nature 221, 1241–1244 (1969). https://doi.org/10.1038/2211241a0
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DOI: https://doi.org/10.1038/2211241a0
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