Abstract
ELECTROPHORETIC migration of proteins depends on their electrophoretic mobility and the resistance of the supporting medium. The latter is an important factor in starch and polyacrylamide gels the action of which as molecular sieves contributes greatly to the resolution of protein mixtures. This is further enhanced in gradient gels1–6 in which protein molecules are driven through progressively decreasing pores until they are brought to a near dead stop in order of their size; a procedure appropriately called “pore-limit electrophoresis”5.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Margolis, J., and Kenrick, K. G., Nature, 214, 1334 (1967).
Margolis, J., and Kenrick, K. G., Biochem. Biophys. Res. Commun., 27, 68 (1967).
Margolis, J., and Kenrick, K. G., Anal. Biochem., 25, 347 (1968).
Slater, G. G., Fed. Proc., 24, 225 (1965).
Slater, G. G., Anal. Biochem., 24, 215 (1968).
Thorun, W., and Mehl, E., Biochim. Biophys. Acta, 160, 132 (1968).
Smithies, O., and Poulik, M. D., Nature, 177, 1033 (1956).
Raymond, S., Ann. NY Acad. Sci., 121, 351 (1964).
Ornstein, L., Ann. NY Acad. Sci., 121, 321 (1964).
Davis, B. J., Ann. NY Acad. Sci., 121, 404 (1964).
Fazekas de St. Groth, S., Webster, R. G., and Datyner, A., Biochim. Biophys. Acta, 71, 377 (1963).
Margolis, J., Anal. Biochem. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MARGOLIS, J., KENRICK, K. Two-dimensional Resolution of Plasma Proteins by Combination of Polyacrylamide Disc and Gradient Gel Electrophoresis. Nature 221, 1056–1057 (1969). https://doi.org/10.1038/2211056a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2211056a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.