Abstract
ALTHOUGH chlorophyll c has been purified by several different methods1–3, its reported spectral properties vary widely. The ratios of Soret (450 nm) to red (630 nm) bands of chlorophyll c in diethyl ether have been given as 7.0 (ref. 4), 8.9 (ref. 2), 11.4 (ref. 1), 11.5 (ref. 3) and 12.5 (ref. 5). Some of these differences in band ratios can now be explained by the finding of two spectrally distinct components in preparations of chlorophyll c. In addition, different marine algae contain different proportions of the two components. Each component has a three-banded chlorophyll c spectrum which differs markedly in band ratios, with, however, only small differences in the position of the absorption maxima. Separation of the two components was achieved on thin layers of a special polyethylene powder (Dow Chemical Co., melt index <2, particle size about 1 micron).
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References
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JEFFREY, S. Two Spectrally Distinct Components in Preparations of Chlorophyll c. Nature 220, 1032–1033 (1968). https://doi.org/10.1038/2201032a0
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DOI: https://doi.org/10.1038/2201032a0
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