Article | Published:

Function of Three Protein Factors and Ribosomal Subunits in the Initiation of Protein Synthesis in E. coli

Naturevolume 219pages10161021 (1968) | Download Citation

Subjects

Abstract

A study of the effects of the three initiation factors on the formation of the initiation complex has shown that factor C promotes AUG dependent binding of F-met-tRNAF to the free 30S ribosomal subunit, while factor A is required as well as factor C for the formation of the 70S-AUG-F-met-tRNAF complex. When natural mRNA is used as template, factor B has to be added to obtain binding of F-met-tRNAF in a configuration which allows peptide bond formation.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Marcker, K. A., and Sanger, F., J. Mol. Biol., 8, 835 (1964).

  2. 2

    Adams, J. M., and Capecchi, M. R., Proc. US Nat. Acad. Sci., 55, 147 (1966).

  3. 3

    Clark, B. F. C., and Marcker, K. A., J. Mol. Biol., 17, 394 (1966).

  4. 4

    Eisenstadt, J. M., and Brawerman, G., Biochemistry, 5, 2777 (1966).

  5. 5

    Stanley, W. M., Salas, M., Wahba, A. J., and Ochoa, S., Proc. US Nat. Acad. Sci., 56, 290 (1966).

  6. 6

    Revel, M., and Gros, F., Biochem. Biophys. Res. Commun., 25, 124 (1966).

  7. 7

    Mangiarotti, G., and Schlessinger, D., J. Mol. Biol., 20, 123 (1966).

  8. 8

    Kaempfer, R., Meselson, M., and Raskas, M., J. Mol. Biol., 31, 277 (1968).

  9. 9

    Eisenstadt, J., and Brawerman, G., Proc. US Nat. Acad. Sci., 58, 1560 (1967).

  10. 10

    Revel, M., and Gros, F., Biochem. Biophys. Res. Commun., 27, 12 (1967).

  11. 11

    Revel, M., Herzberg, M., Becarevic, A., and Gros, F., J. Mol. Biol., 33, 231 (1968).

  12. 12

    Babinet, C., Biochem. Biophys. Res. Commun., 26, 639 (1967).

  13. 13

    Salser, W., Gesteland, W., and Bolle, M. A., Nature, 215, 588 (1967).

  14. 14

    Lucas Lenard, J., and Lipmann, F., Proc. US Nat. Acad. Sci., 57, 1050 (1967).

  15. 15

    Nomura, M., and Lowry, C. V., Proc. US Nat. Acad. Sci., 58, 946 (1967).

  16. 16

    Grunberg-Manago, M., Revel, M., Dondon, J., Hayes, D., and Gros, F., Bull. Soc. Chim. Biol. (in the press).

  17. 17

    Bretscher, M. S., and Marcker, K. A., Nature, 211, 380 (1966).

  18. 18

    Iwasaki, K., Sabol, S., Wahba, A., and Ochoa, S., Arch. Biochem. Biophys. (in the press).

  19. 19

    Maitra, U., and Doubnoff, J., Fed. Proc., 27, 398 (1968).

  20. 20

    Brown, J. C., and Doty, P., Biochem. Biophys. Res. Commun., 30, 284 (1968).

  21. 21

    Mukundan, N., Hershey, J., Dewey, K., and Thach, R., Nature, 217, 1013 (1968).

  22. 22

    Hille, M. B., Miller, M. J., Iwasaki, K., and Wahba, A., Proc. US Nat. Acad. Sci., 58, 1652 (1967).

  23. 23

    Lodish, H., J. Mol. Biol. (in the press).

  24. 24

    Nirenberg, M., and Leder, P., Science, 145, 1399 (1964).

  25. 25

    Leder, P., and Burztyn, H., Biochem. Biophys. Res. Commun., 25, 233 (1966).

Download references

Author information

Author notes

  1. G. BRAWERMAN: On leave of absence from Department of Biochemistry, Yale University School of Medicine.

Affiliations

  1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, Paris

    • M. REVEL
    • , J. C. LELONG
    • , G. BRAWERMAN
    •  & F. GROS

Authors

  1. Search for M. REVEL in:

  2. Search for J. C. LELONG in:

  3. Search for G. BRAWERMAN in:

  4. Search for F. GROS in:

About this article

Publication history

Received

Issue Date

DOI

https://doi.org/10.1038/2191016a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.