Function of Three Protein Factors and Ribosomal Subunits in the Initiation of Protein Synthesis in E. coli

Abstract

A study of the effects of the three initiation factors on the formation of the initiation complex has shown that factor C promotes AUG dependent binding of F-met-tRNAF to the free 30S ribosomal subunit, while factor A is required as well as factor C for the formation of the 70S-AUG-F-met-tRNAF complex. When natural mRNA is used as template, factor B has to be added to obtain binding of F-met-tRNAF in a configuration which allows peptide bond formation.

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REVEL, M., LELONG, J., BRAWERMAN, G. et al. Function of Three Protein Factors and Ribosomal Subunits in the Initiation of Protein Synthesis in E. coli. Nature 219, 1016–1021 (1968). https://doi.org/10.1038/2191016a0

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