High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea

Abstract

VIRUS-SPECIFIC non-structural antigens (T antigens) are produced early in the lytic cycle of adenovirus infection and are also found in cells transformed by adenovirus1–3. Synthesis of T antigen, unlike that of the structural antigen, is not dependent on DNA synthesis; for example, fluorodeoxyuridine (FUdR) effectively inhibits the synthesis of viral antigen (V) without markedly suppressing the production of T antigen4,5. Our studies have been designed to produce large quantities of T antigen “free” from viral structural antigen by manipulation of the lytic interaction of adenoviruses in KB cells.

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LEVY, J., HUEBNER, R., KERN, J. et al. High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea. Nature 217, 744–745 (1968). https://doi.org/10.1038/217744a0

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