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High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea

Nature volume 217, pages 744–745 (1968)Cite this article

Abstract

VIRUS-SPECIFIC non-structural antigens (T antigens) are produced early in the lytic cycle of adenovirus infection and are also found in cells transformed by adenovirus1–3. Synthesis of T antigen, unlike that of the structural antigen, is not dependent on DNA synthesis; for example, fluorodeoxyuridine (FUdR) effectively inhibits the synthesis of viral antigen (V) without markedly suppressing the production of T antigen4,5. Our studies have been designed to produce large quantities of T antigen “free” from viral structural antigen by manipulation of the lytic interaction of adenoviruses in KB cells.

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Authors and Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

    JAY A. LEVY & ROBERT J. HUEBNER

  2. Flow Laboratories Incorporated, Rockville, Maryland

    JEROME KERN & RAYMOND V. GILDEN

Authors
  1. JAY A. LEVY
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  2. ROBERT J. HUEBNER
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  3. JEROME KERN
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  4. RAYMOND V. GILDEN
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LEVY, J., HUEBNER, R., KERN, J. et al. High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea. Nature 217, 744–745 (1968). https://doi.org/10.1038/217744a0

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