High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea

Nature volume 217, pages744–745(1968)Cite this article

Abstract

VIRUS-SPECIFIC non-structural antigens (T antigens) are produced early in the lytic cycle of adenovirus infection and are also found in cells transformed by adenovirus1–3. Synthesis of T antigen, unlike that of the structural antigen, is not dependent on DNA synthesis; for example, fluorodeoxyuridine (FUdR) effectively inhibits the synthesis of viral antigen (V) without markedly suppressing the production of T antigen4,5. Our studies have been designed to produce large quantities of T antigen “free” from viral structural antigen by manipulation of the lytic interaction of adenoviruses in KB cells.

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References

  1. 1

    Hoggan, M. D., Rowe, W. P., Black, P. H., and Huebner, R. J., Proc. US Nat. Acad. Sci., 53, 12 (1965).

    ADS  Article  CAS  Google Scholar 

  2. 2

    Huebner, R. J., Rowe, W. P., Turner, H. C., and Lane, W. T., Proc. US Nat. Acad. Sci., 50, 379 (1963).

    ADS  Article  CAS  Google Scholar 

  3. 3

    Pope, J. H., and Rowe, W. P., J. Exp. Med., 120, 577 (1964).

    Article  CAS  Google Scholar 

  4. 4

    Gilead, Z., and Ginsberg, H. S., J. Bacteriol., 90, 120 (1965).

    PubMed  PubMed Central  CAS  Google Scholar 

  5. 5

    Lewis, jun., A. M., Wiese, W. H., and Rowe, W. P., Proc. US Nat. Acad. Sci., 57, 622 (1967).

    ADS  Article  CAS  Google Scholar 

  6. 6

    Gilden, R. V., Kern, J., Lee, Y. K., and Huebner, R. J., Proc. Soc. Exp. Biol. and Med. (in the press).

  7. 7

    Rosenkranz, H. S., and Levy, J. A., Biochim. Biophys. Acta, 95, 181 (1965).

    Article  CAS  Google Scholar 

  8. 8

    Rosenkranz, H. S., Garro, A. J., Levy, J. A., and Carr, H. S., Biochim. Biophys. Acta, 114, 501 (1966).

    Article  CAS  Google Scholar 

  9. 9

    Rosenkranz, H. S., Rose, H. M., Morgan, C., and Hsu, K. C., Virology, 28, 510 (1966).

    Article  CAS  Google Scholar 

  10. 10

    Sever, J. L., J. Immunol., 88, 320 (1962).

    PubMed  CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

    JAY A. LEVY & ROBERT J. HUEBNER

  2. Flow Laboratories Incorporated, Rockville, Maryland

    JEROME KERN & RAYMOND V. GILDEN

Authors
  1. JAY A. LEVY
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  2. ROBERT J. HUEBNER
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  3. JEROME KERN
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  4. RAYMOND V. GILDEN
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LEVY, J., HUEBNER, R., KERN, J. et al. High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea. Nature 217, 744–745 (1968). https://doi.org/10.1038/217744a0

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