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Hox genes in brachiopods and priapulids and protostome evolution

Abstract

Understanding the early evolution of animal body plans requires knowledge both of metazoan phylogeny and of the genetic and developmental changes involved in the emergence of particular forms. Recent 18S ribosomal RNA phylogenies suggest a three-branched tree for the Bilateria comprising the deuterostomes and two great protostome clades, the lophotrochozoans1 and ecdysozoans2. Here, we show that the complement of Hox genes in critical protostome phyla reflects these phylogenetic relationships and reveals the early evolution of developmental regulatory potential in bilaterians. We have identified Hox genes that are shared by subsets of protostome phyla. These include a diverged pair of posterior (Abdominal-B -like) genes in both a brachiopod and a polychaete annelid, which supports the lophotrochozoan assemblage, and a distinct posterior Hox gene shared by a priapulid, a nematode and the arthropods, which supports the ecdysozoan clade. The ancestors of each of these two major protostome lineages had a minimum of eight to ten Hox genes. The major period of Hox gene expansion and diversification thus occurred before the radiation of each of the three great bilaterian clades.

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Figure 1: Alignment of Hox homeodomains and flanking sequences.
Figure 2: Phylogenetic analysis of bilaterian posterior Hox homeodomain sequences.
Figure 3: Distribution of Hox genes in bilaterians.

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Acknowledgements

We thank B. Winnepenninckx for L. anatina DNA; A. Knowlton, R. Highsmith, and P. Reynolds for P. caudatus tissue; E. Davidson for his interest and for sharing unpublished data; V. Kassner and N. Lartillot for their help; and A. Friday and G. Budd for comments on the manuscript. This work was supported by grants from the BBSRC and the Wellcome trust (M.E.A.); the Russian Basic Research Foundation and the Royal Society (T.A.); the CNRS, program ‘Genome’ and the Université Paris-Sud (A.A.); and the Howard Hughes Medical Institute (J.K.G. and S.B.C.).

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Correspondence to Guillaume Balavoine.

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de Rosa, R., Grenier, J., Andreeva, T. et al. Hox genes in brachiopods and priapulids and protostome evolution. Nature 399, 772–776 (1999). https://doi.org/10.1038/21631

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