Abstract
BLOOD platelets of various species contain particular, dense, osmiophilic organelles (dense bodies) which are somewhat smaller (500 to 2200 Å) than the α-granules. By combining electron microscopy and 5-hydroxytryptamine determinations, including experiments with reserpine, we have provided strong evidence that the osmiophility of the organelles is caused by an accumulation of 5-hydroxytryptamine1–4. Experiments with tyramine confirmed this view5. Here, we report attempts to isolate the dense osmiophilic bodies of platelets by density gradient centrifugation. Furthermore, the distribution of 5-hydroxytryptamine and histamine in various subcellular platelet fractions, including the dense bodies, was determined.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Tranzer, J. P., Da Prada, M., and Pletscher, A., Nature, 212, 1574 (1966).
Tranzer, J. P., Pletscher, A., and Da Prada, M., Helv. Physiol. Acta, 24, C108 (1966).
Da Prada, M., Tranzer, J. P., and Pletscher, A., J. Pharmacol. Exp. Therap (in the press).
Tranzer, J. P., Da Prada, M., and Pletscher, A., in Advances in Pharmacology (Academic Press, in the press).
Bak, I. J., Hassler, R., May, B., and Westermann, E., Life Sci., 6, 1133 (1967).
Bartholini, G., Pletscher, A., and Gey, K. F., Experientia, 17, 541 (1961).
Bogdanski, D. F., Pletscher, A., Brodie, B. B., and Udenfriend, S., J. Pharmacol. Exp. Therap., 117, 82 (1956).
Huff, J. A., Davis, V. E., and Brown, H., J. Lab. Clin. Med., 67, 1044 (1966).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Maynert, E. W., and Isaac, L., in Advances in Pharmacology (Academic Press, in the press).
Solatunturi, E., and Paasonen, M. K., Ann. Med. Exp. Fenn., 44, 427 (1966)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DA PRADA, M., PLETSCHER, A., TRANZER, J. et al. Subcellular Localization of 5-Hydroxytryptamine and Histamine in Blood Platelets. Nature 216, 1315–1317 (1967). https://doi.org/10.1038/2161315a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2161315a0
This article is cited by
-
Platelets as a model for neurones?
Experientia (1988)
-
Isolation and partial characterization of platelet α-granule membranes
The Journal of Membrane Biology (1983)
-
Cyclic AMP of blood platelets: Accumulation in organelles storing 5-hydroxytryptamine and ATP
Experientia (1972)
-
New aspects on the storage of 5-hydroxytryptamine in blood platelets
Experientia (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.