Novel Approach to studying Relationships between Mycoplasmas and Tissue Culture Cells

Abstract

MOST continuous cultures of tissue cells are contaminated with mycoplasmas1, which can be detected by staining2, immunofluorescence3, isolation3 or biochemical studies4. Furthermore, the interaction of mycoplasma organisms with HeLa cells and other types of mammalian cells has been studied with the electron microscope5,6. Electron microscopy, in particular, shows that there is an intimate relationship between mycoplasmas and infected cells, which might explain why it is so difficult to eliminate these organisms from tissue culture cell lines and strains.

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TAYLOR-ROBINSON, D., MANCHEE, R. Novel Approach to studying Relationships between Mycoplasmas and Tissue Culture Cells. Nature 216, 1306–1307 (1967). https://doi.org/10.1038/2161306a0

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