Abstract
WHEN lymphoid cells from spleen, lymph nodes or thoracic duct lymph are transferred together with sheep red cells to heavily irradiated recipients, an immune response occurs, the magnitude of which is related to the number of lymphoid cells injected1–3. Although Playfair1 and Kennedy2 have described quantitative methods for determining the number of cells which are sensitive to antigens in such an inoculum, very little is known about the nature of these cells. The indications are that they are only slowly dividing or that most of them are in a resting state in the non-immunized animal4. Morphological evidence has been presented which tends to implicate small lymphocytes as cells which are sensitive to antigen, but the data are, as yet, unconvincing5,6. The purpose of this work has been to use a different approach in characterizing these cells. The technique is that of sedimentation to equilibrium in density gradients of bovine serum albumin7,8.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Playfair, J. H. L., Papermaster, B. W., and Cole, L. J., Science, 149, 998 (1965).
Kennedy, J. C., Siminovitch, L., Till, J. E., and McCulloch, E. A., Proc. Soc. Exp. Biol., 120, 868 (1965).
Miller, J. F. A. P., and Mitchell, G. F., Nature, 214, 994 (1967).
Syeklocha, D., Siminovitch, L., Till, J. E., and McCulloch, E. A., J. Immunol., 96, 472 (1966).
Gowans, J. L., and McGregor, D. D., Prog. Allergy, 9, 1 (1965).
Nossal, G. J. V., Shortman, K., Miller, J. F. A. P., Mitchell, G. F., and Haskill, J. S., Cold Spring Harb. Symp. Quant. biol. (in the press).
Shortman, K., Haskill, J. S., Szenberg, A., and Legge, D. G. (preceding communication).
Shortman, K., Austral. J. Exp. Biol. Med. Sci. (in the press).
Jerne, N. K., and Nordin, A. A., Science, 140, 405 (1963).
Albright, J. F., and Makinodan, T., Molecular and Cellular Basis of Antibody Formation (edit. by Sterzl, J.), 427 (Czechoslovak Academy of Sciences, Prague, 1965).
Ford, W. L., Gowans, J. L., and McCullagh, P. J., The Thymus: Experimental and Clinical Studies (edit. by Wolstenholme, G. E. W., and Porter, R.), 58 (Ciba Symposium, 1966, Churchill Ltd., London).
Bruchovsky, N., Owen, A. A., Becker, A. J., and Till, T. E., Cancer Res., 25, 1232 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HASKILL, J. Density Distribution Analysis of Antigen Sensitive Cells in the Rat. Nature 216, 1229–1231 (1967). https://doi.org/10.1038/2161229a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/2161229a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.