Rapid Isolation of Active Mitochondria from Plant Tissue

Abstract

MOST of the methods which have been described for the isolation of mitochondria from a variety of tissues1–3 are essentially very similar. The cells are gently disrupted in a suitable medium, the homogenate is then filtered through a coarse filter and the supernatant obtained is fractionated into nuclear, mitochondrial, microsomal(+) soluble components by differential centri-fugation. The mitochondrial fraction, which is obtained by centrifugation at 5,000–15,000g for 15–30 min, is then washed and resedimented. One of the drawbacks of this conventional technique is that it is both time and labour consuming, making it impractical to isolate mitochondria from several different sources (for example, tissue receiving different experimental treatments) on the same day, or to compare the effects of different buffers, in the isolation media, on the subsequent activity of the mitochondria.

References

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    Wedding, R. T., and Black, M. K., Plant Physiol., 37, 364 (1962).

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    Nobel, P. S., Plant Physiol. (in the press).

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PALMER, J. Rapid Isolation of Active Mitochondria from Plant Tissue. Nature 216, 1208 (1967). https://doi.org/10.1038/2161208a0

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