Molecular Weight of Human Follicle Stimulating Hormone


FOLLICLE stimulating hormone (FSH) was obtained from powdered human pituitary dried with acetone and purified in two ways: (a) by the method of Amir, Barker, Butt and Crooke1, and (b) by the cellosolve extraction procedure of Butt, Crooke and Wolf2 followed by fractionation on DEAE-‘Sephadex A-50’. Haemagglutination inhibition tests showed that luteinizing hormone (LH) and albumin were present to an extent of less than 1 per cent by weight of protein. FSH obtained by both procedures seems to undergo reversible association and dissociation, as the following experiments show.

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  1. 1

    Amir, S. M., Barker, S. A., Butt, W. R., and Crooke, A. C., Nature, 209, 1092 (1966).

  2. 2

    Butt, W. R., Crooke, A. C., and Wolf, A., in Gonadotropins: Physicochemical and Immunological Properties, Ciba Foundation Study Group, No. 22 (edit. by Wolstenholme, G. E. W.), 85 (J. and A. Churchill, Ltd., London, 1965).

  3. 3

    Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961).

  4. 4

    Odell, W. D., Swain, R. W., and Nydick, M., J. Clin. Endocrinol. Metab., 24 (12), 1266 (1964).

  5. 5

    Li, C. H., and Starman, B., Nature, 202, 291 (1964).

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GRAY, C. Molecular Weight of Human Follicle Stimulating Hormone. Nature 216, 1112–1113 (1967).

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