Relevance of Proton Uptake induced by Light to the Mechanism of Energy Coupling in Photophosphorylation

Abstract

THE relevance of proton movements which are dependent on energy to energy conservation mechanisms in oxidative phosphorylation and photophosphorylation has recently been extensively debated, particularly with respect to the “chemiosmotic”1,2 and chemical3,4 hypotheses. There are a few critical tests of the chemiosmotic theory. These include: (a) the ratio of protons taken up to electrons transported; the value of this H+: e ratio should equal the number of sites of energy conservation in the election transport path; (b) the effect of phosphorylating conditions; during phosphorylation one should observe a fall in the steady state extent of the proton gradient, as a result of the stoichiometric utilization of one or two protons2 per ATP synthesized. Reversible pH changes in spinach chloroplasts induced by light have previously been described in detail5,6. This communication reports experiments designed to test the chemiosmotic theory by these criteria. The results cannot be reconciled with the theory as it is at present conceived.

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References

  1. 1

    Mitchell, P., Nature, 191, 144 (1961).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Mitchell, P., Biol. Rev., 41, 445 (1966).

    CAS  Article  Google Scholar 

  3. 3

    Slater, E. C., Nature, 172, 975 (1953).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Slater, E. C., Europ. J. Biochem., 1, 317 (1967).

    CAS  Article  Google Scholar 

  5. 5

    Neumann, J., and Jagendorf, A. T., Arch. Biochem. Biophys., 107, 1 (1964).

    Article  Google Scholar 

  6. 6

    Jagendorf, A. T., and Neumann, J., J. Biol. Chem., 240, 3210 (1965).

    CAS  PubMed  Google Scholar 

  7. 7

    Lynn, W., and Brown, R., J. Biol. Chem., 242, 426 (1967).

    CAS  PubMed  Google Scholar 

  8. 8

    Dilley, R. A., and Vernon, L. P., Proc. US Nat. Acad. Sci., 56, 395 (1967).

    ADS  Article  Google Scholar 

  9. 9

    Nishimura, N., Ito, T., and Chance, B., Biochim. Biophys. Acta, 59, 177 (1962).

    CAS  Article  Google Scholar 

  10. 10

    Avron, M., and Jagendorf, A. T., J. Biol. Chem., 234, 967 (1959).

    CAS  PubMed  Google Scholar 

  11. 11

    Crofts, A. R., Biochim. Biophys. Acta, 131, 97 (1967).

    CAS  Article  Google Scholar 

  12. 12

    McCarty, R. E., Guillory, R. J., and Racker, E., J. Biol. Chem., 240, 4822 (1965).

    CAS  PubMed  Google Scholar 

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KARLISH, S., AVRON, M. Relevance of Proton Uptake induced by Light to the Mechanism of Energy Coupling in Photophosphorylation. Nature 216, 1107–1109 (1967). https://doi.org/10.1038/2161107a0

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