Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Nature of Luteinizing Hormone Releasing Factor in Hypophysial Portal Blood

Nature volume 215, pages 159–161 (1967)Cite this article

Abstract

IN 1966 Worthington1 described a method for the collection of hypophysial portal blood from the cut pituitary stalk of rats. This method was used by Fink, Nallar and Worthington2,3 to demonstrate the presence of luteinizing hormone releasing factor (LRF) in portal blood obtained from rats in proestrus and from rats hypophysectomized at least two weeks before collection. The chief disadvantage of this method of collection of portal blood was that contamination of samples by blood flowing back through pituitary sinusoids and bearing significant quantities of anterior pituitary hormones could not be prevented. Thus, because the ovarian ascorbic acid depletion method4,5 was used to assay the plasma, some of the activity exhibited by portal plasma from animals in proestrus probably resulted from luteinizing hormone (LH).

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Worthington, jun., W. C., Nature, 210, 710 (1966).

    Article  ADS  CAS  Google Scholar 

  2. Fink, G., Nallar, R., and Worthington, jun., W. C., J. Physiol., 183, 20P (1966).

    Google Scholar 

  3. Fink, G., Nallar, R., and Worthington, jun., W. C., J. Physiol. (in the press) (1967).

  4. Parlow, A. F., Fed. Proc., 17, 702 (1958).

    Google Scholar 

  5. McCann, S. M., and Taleisnik, S., Amer. J. Physiol., 199, 847 (1960).

    Article  CAS  Google Scholar 

  6. Fink, G., J. Physiol. (in the press).

  7. Harris, G. W., Reed, M., and Fawcett, C. P., Brit. Med. Bull., 22, 266 (1966).

    Article  CAS  Google Scholar 

  8. Ramirez, V. D., and McCann, S. M., Endocrinology, 73, 193 (1963).

    Article  CAS  Google Scholar 

  9. Gellote, B., J. Chromatog., 3, 330 (1960).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Anatomy, University of Oxford,

    GEORGE FINK

Authors
  1. GEORGE FINK
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

FINK, G. Nature of Luteinizing Hormone Releasing Factor in Hypophysial Portal Blood. Nature 215, 159–161 (1967). https://doi.org/10.1038/215159a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/215159a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing