Multiple Budding on Carrot Embryos arising in Tissue Culture

Abstract

STEWARD et al.¹ reported the development in culture of carrot embryos which appeared normal and were able to continue development through all stages of the normal carrot life cycle. Waris², on the other hand, has reported that embryos from the seeds of Oenanthe aquatica L., in the presence of 0.1 per cent glycine, enter an abnormal developmental cycle in which nodules form on the embryo, then break off to form further embryos; most of the embryos were of abnormal structure. We have observed carrot embryoids of abnormal structure which were developed in tissue culture; they budded off other embryoids instead of continuing their development towards plantlets.