Abstract
THE extreme heterogeneity of the bone marrow in which leucocytes and erythrocytes develop has impeded studies of the biochemical changes accompanying the growth and maturation of these cells. All stages of their development are intermingled in this tissue, together with other cell types. Centrifugation in density gradients, exploiting differences in sedimentation rate1 and in buoyant density2,3, electrophoresis in specially designed apparatus4, or sedimentation at 1g in a density-stabilized system of horizontally flowing liquid layers5 have achieved the separation of myeloid cells from erythroid but provided little or no resolution of the maturing cells within these series.
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References
Goodman, J. W., Exp. Cell Res., 21, 88 (1960).
Tsuji, K., Osaka City Med. J., 8, 91 (1962).
Hilal, S. K., Mosser, D. G., Loken, M. K., and Johnson, R. W., Ann. N.Y. Acad. Sci., 114, 661 (1964).
Ruhenstroth-Bauer, G., in Jahrbuch der Max-Planck-Gesellschaft zur Förderung der Wissenschaften, 108 (Hubert and Co., Göttingen, 1964).
Mel, H. C., Mitchell, L. T., and Thorell, B., Blood, 25, 63 (1965).
Mel, H. C., J. Theoret. Biol., 6, 181 (1964).
Yoffey, J. M., Hudson, G., and Osmond, D. G., J. Anat., 99, 841 (1965).
Wintrobe, M. M., in Clinical Hematology, 53 (Lea and Febiger, Philadelphia, 1961).
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PETERSON, E., EVANS, W. Separation of Bone Marrow Cells by Sedimentation at Unit Gravity. Nature 214, 824–825 (1967). https://doi.org/10.1038/214824a0
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DOI: https://doi.org/10.1038/214824a0
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