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Studies of the Distribution of Haemoglobin E, Thalassaemias and Glucose-6-phosphate Dehydrogenase Deficiency in North-eastern Thailand

Naturevolume 214pages501502 (1967) | Download Citation



DURING our survey in 1963, examinations were made of 1,030 subjects in the provinces of Ubol(rajthani), Khonkaen and Udorn(thani) in north-eastern Thailand. The subjects were villagers, soldiers, hospital patients and prisoners. Haemoglobin types were based on cellulose acetate strip electrophoresis in veronal buffer, pH. 8.6, ionic strength 0.025. Thalassaemia trait was designated to those with Hb A who showed decreased red cell osmotic fragility in a one tube screening method1, hypochromic microcytic red cells and serum iron not lower than 50γ per cent; these persons who had Hb A2, as determined by starch block electrophoresis2,3, higher than 3.1 per cent were further classified as β-thalassaemia traits and the others with 3.1 per cent Hb A2 or lower as α-thalassaemia traits. In Ubol glucose-6-phosphate dehydrogenase (G-6-PD) activity was screened with Motulsky's technique4, but in Khonkaen and Udorn a spot test was carried out according to Fairbanks and Beutler5.

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  1. Division of Haematology, Department of Medicine, Faculty of Medicine and Siriraj Hospital, Bangkok, Thailand

    • P. WASI
    • , S. NA-NAKORN


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