Abstract
THE letter of Bianchi and de Bianchi1 prompts us to comment on our own experience in preparing slides for the study of sex chromatin in amniotic membrane cells from rat foetuses. They mentioned in their letter the difficulty of fixing the amniotic membrane of rat foetuses with absolute or 95 per cent alcohol, resulting in coagulation of mucopolysaccharides and shrinkage of the cells.
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References
Bianchi, N. E., and de Bianchi, M. S. A., Nature, 212, 1593 (1966).
Sanderson, A. R., and Stewart, J. S. S., Brit. Med. J., ii, 1065 (1961).
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FARÍAS, E., KAJII, T. & GARDNER, L. Technique for Investigation of Sex Chromatin in Amniotic Membrane of Rat Foetuses. Nature 214, 499–500 (1967). https://doi.org/10.1038/214499b0
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DOI: https://doi.org/10.1038/214499b0
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