Letter | Published:

Proposed Technique for classifying and identifying Encephalitogens

Naturevolume 214pages407408 (1967) | Download Citation



EXPERIMENTAL allergic encephalomyelitis (EAE) can be produced in guinea-pigs by injection of acid extracts of central nervous tissue mixed with Freund's complete adjuvant. Some authors claim that the active factor in these extracts is protein1–4 while others claim that it can be polypeptide5–9. From extracts of ox spinal cord we have isolated a bovine encephalitogenic polypeptide fraction (BEP) and also an encephalitogenic protein fraction. Similar fractions were also isolated from extracts of guinea-pig brain. As other encephalitogens may be isolated in the future from nervous tissue of various species it would be important to have a simple means of identifying and classifying them. At present it is difficult to correlate the results of chemical and immunological expariments from different laboratories because authors often fail to describe adequately the nature and homogeneity of their encephalitogenic preparations. This paper describes a simple gel electrophoresis procedure which can test the homogeneity of encephalitogenic preparations and provides a convenient classification of the proteins or polypeptides in such materials. The proposed classification is based on a comparison of the mobility of the encephalitogen relative to the mobility of cytcochrome c.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Kies, M. W., Ann. N.Y. Acad. Sci., 122, 161 (1965).

  2. 2

    Wolfgram, F., Ann. N.Y. Acad. Sci., 122, 104 (1965).

  3. 3

    Caspary, E. A., and Field, E. J., Ann. N.Y. Acad. Sci., 122, 182 (1965).

  4. 4

    Kibler, R. F., Fox, R. H., and Shapira, R., Nature, 204, 1273 (1964).

  5. 5

    Robertson, D. M., Blight, R., and Lumsden, C. E., Nature, 196, 1005 (1962).

  6. 6

    Lumsden, C. E., Robertson, D. M., and Blight, R., J. Neurochem., 113, 127 (1966).

  7. 7

    Nakao, A., and Roboz-Einstein, E., Ann. N.Y. Acad. Sci., 122, 171 (1965).

  8. 8

    Kongruth, S. E., and Anderson, J. W., J. Cell Biol., 26, 157 (1965).

  9. 9

    Carnegie, P. R., and Lumsden, C. E., Nature, 209, 1354 (1966).

  10. 10

    Driedger, A., Johnson, L. D., and Marko, A. M., Can. J. Biochem. Physiol., 41, 2507 (1963).

  11. 11

    Schonne, E., Protides of the Biological Fluids, 11, 368 (1964).

  12. 12

    Kies, M. W., Alvord, E. C., Martenson, R. E., and LeBaron, F. N., Science, 151, 821 (1966).

  13. 13

    Kies, M. W., Proc. Fifth Intern. Cong. Neuropathology, Zurich, 1965, 257 (Excerpta Medica, Amsterdam, 1966).

  14. 14

    Bagdasarian, M., Matheson, N. A., Synge, R. L. M., and Youngson, M. A., Biochem. J., 91, 91 (1964).

Download references

Author information


  1. The Russell Grimwade School of Biochemistry and the Clinical Research Unit of the Walter and Eliza Hall Institute, University of Melbourne, Parkville, N.2, Victoria, Australia

    • P. R. CARNEGIE
    • , G. LAMOUREUX
    •  & B. BENCINA


  1. Search for P. R. CARNEGIE in:

  2. Search for G. LAMOUREUX in:

  3. Search for B. BENCINA in:

About this article

Publication history



Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.