Letter | Published:

Differences in Uridine Diphosphate Galactose-4-Epimerase between Haemolysates of Newborns and of Adults

Naturevolume 214pages283284 (1967) | Download Citation

Subjects

Abstract

THIS communication presents an observation not yet reported concerning the effect of exogenous nicotinamide adenine dinucleotide (NAD) on the activity of uridine diphosphate galactose-4-epimerase. This enzyme (formerly called galactowaldenase) was first demonstrated in yeast extract by Leloir1. NAD was shown later to be required for activity in a calf liver preparation2. The need for an exogenous supply of NAD was further shown in crude human haemolysates3 and in homogenates of L cells4. It now has been found that haemolysates from newborn infants demonstrate a substantial epimerase activity without the addition of NAD.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Leloir, L. F., Arch. Biochem. Biophys., 33, 186 (1951).

  2. 2

    Maxwell, E. S., J. Amer. Chem. Soc., 78, 1074 (1956).

  3. 3

    Isselbacher, K. J., Anderson, E. P., Kurahashi, K., and Kalckar, H. M., Science, 123, 635 (1956).

  4. 4

    Robinson, E. A., Kalckar, H. M., and Troedsson, H., Biochem. Biophys. Res. Commun., 13, 313 (1963).

  5. 5

    Ng, W. G., Donnell, G. N., and Bergren, W. R., J. Lab. Clin. Med., 66, 115 (1965).

  6. 6

    Kabacy, G., Hodgman, J., Donnell, G. N., and Spears, R., Pediatrics, 38, 125 (1966).

  7. 7

    Horecker, B. L., and Kornberg, A., J. Biol. Chem., 175, 385 (1948).

  8. 8

    Gross, R. T., Schroeder, E. A., and Gabrio, B. W., J. Clin. Invest., 45, 249 (1966).

  9. 9

    Rubinstein, E., Ottolenghi, P., and Denstedt, O. F., Canad. J. Biochem. Physiol., 34, 222 (1956).

  10. 10

    Gasiorowska, I., and Racznska-Bojanowska, K., Bull. Acad. Polon. Sci., 11, 417 (1963).

Download references

Author information

Affiliations

  1. Departments of Biochemistry and of Pediatrics of the School of Medicine, University of Southern California. Children's Hospital of Los Angeles. Los Angeles County General Hospital

    • WON G. NG
    • , GEORGE N. DONNELL
    • , JOAN E. HODGMAN
    •  & WILLIAM R. BERGREN

Authors

  1. Search for WON G. NG in:

  2. Search for GEORGE N. DONNELL in:

  3. Search for JOAN E. HODGMAN in:

  4. Search for WILLIAM R. BERGREN in:

About this article

Publication history

Issue Date

DOI

https://doi.org/10.1038/214283a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.