Letter | Published:

Metabolite-promoted Heat Lability of β-Galactosidase and its Relation to Catabolite Repression

Naturevolume 214pages272273 (1967) | Download Citation

Subjects

Abstract

Gest and Mandelstam have reported1 that β-galactosidase from Escherichia coli is more heat labile in the presence of fructose-1,6-diphosphate (FDP) than in its absence. They found the effect to be quite specific: of the other sugars and phosphorylated esters tested, only glucose-6-phosphate (G6P) weakly, but significantly, increased the heat lability of the enzyme. These results support the possibility that β-galactosidase possesses a specific binding site for FDP. Gest and Mandelstam1 suggested that FDP, which is a key intermediate in carbohydrate metabolism, might be the repressor metabolite postulated by Vogel2 and Szilard3. If this were true, catabolite repression would be effected by the production of FDP, which would then combine with nascent β-galactosidase to prevent the enzyme from leaving the ribosomes on which it is being synthesized.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gest, H., and Mandelstam, J., Nature, 211, 72 (1966).

  2. 2

    Vogel, H. J., Proc. U.S. Nat. Acad. Sci., 43, 491 (1957).

  3. 3

    Szilard, L., Proc. U.S. Nat. Acad. Sci., 46, 271 (1960).

  4. 4

    Prevost, C., and Moses, V., Biochem. J. (in the press).

  5. 5

    Moses, V., and Prevost, C., Biochem. J., 100, 336 (1966).

  6. 6

    Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

  7. 7

    Palmer, J., and Moses, V., Biochem. J. (in the press).

Download references

Author information

Affiliations

  1. Lawrence Radiation Laboratory, University of California, Berkeley, California

    • MARY E. BREWER
    •  & V. MOSES

Authors

  1. Search for MARY E. BREWER in:

  2. Search for V. MOSES in:

About this article

Publication history

Received

Revised

Issue Date

DOI

https://doi.org/10.1038/214272a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.