Abstract
Genetic experiments show that a group of genes may be switched off by the product of a regulator gene, called a repressor. An isolated repressor is shown here to bind specifically and with high affinity to DNA, strongly suggesting that, in vivo, repressors block the transcription from DNA to RNA by binding directly to the DNA.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).
Gilbert, W., and Mueller-Hill, B., Proc. U.S. Nat. Acad. Sci., 56, 1891 (1966).
Ptashne, M., Proc. U.S. Nat. Acad. Sci., 57, 306 (1967).
Kaiser, A. D., and Jacob, F., Virology, 4, 509 (1957).
Isaacs, L. N., Echols, H., and Sly, W. S., J. Mol. Biol., 13, 963 (1965).
Jacob, F., and Campbell, A., C.R. Acad. Sci., Paris, 248, 3219 (1959).
Jacob, F., Sussman, R., and Monod, J., C.R. Acad. Sci., Paris, 254, 4214 (1962).
Sadler, J. R., and Novick, A., J. Mol. Biol., 12, 305 (1965).
Thomas, jun., C. A., and Abelson, J., Procedures in Nucleic Acid Research, edited by Cantoni, G. L., and Davies, D. R., 553 (Harper and Row, New York, 1966).
Modified according to E. Kennedy, pers. comm., from Patterson, M. S., and Greene, R. C., Ann. Chem., 37, 854 (1965).
Studier, F. W., J. Mol. Biol., 11 373 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PTASHNE, M. Specific Binding of the λ Phage Repressor to λ DNA. Nature 214, 232–234 (1967). https://doi.org/10.1038/214232a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/214232a0
This article is cited by
-
A computational model of stem cells’ internal mechanism to recapitulate spatial patterning and maintain the self-organized pattern in the homeostasis state
Scientific Reports (2024)
-
A computational model of stem cells’ decision-making mechanism to maintain tissue homeostasis and organization in the presence of stochasticity
Scientific Reports (2022)
-
Sorghum breeding in the genomic era: opportunities and challenges
Theoretical and Applied Genetics (2021)
-
Redefining fundamental concepts of transcription initiation in bacteria
Nature Reviews Genetics (2020)
-
Towards a comprehensive catalogue of validated and target-linked human enhancers
Nature Reviews Genetics (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.