Abstract
THERE are several theories about the nature of the argentaffin substance stored in the enterochromaffin cells of the gastrointestinal mucosa1–3. At present, Erspamer's 5-hydroxytryptamine theory4,5 appears to be the most popular and best documented. The identification of the enterochromaffin substance as 5-hydroxytryptamine, however, contrasts with the fact that, by using several histochemical methods, no indole reactions have been obtained on formalin-fixed enterochromaffin granules6. We have reconsidered the problem by fixing duodenum, stomach and pancreas of various species, including guinea-pig, rat, mouse, rabbit, horse, pig, monkey and man, with 4 per cent formaldehyde or with 6 per cent glutaraldehyde in 0.1 molar phosphate buffer, pH 7.2, for 24 h.
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SOLCIA, E., SAMPIETRO, R. Indole Nature of Enterochromaffin Substance. Nature 214, 196–197 (1967). https://doi.org/10.1038/214196a0
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DOI: https://doi.org/10.1038/214196a0
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