Letter | Published:

Indole Nature of Enterochromaffin Substance

Naturevolume 214pages196197 (1967) | Download Citation

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Abstract

THERE are several theories about the nature of the argentaffin substance stored in the enterochromaffin cells of the gastrointestinal mucosa1–3. At present, Erspamer's 5-hydroxytryptamine theory4,5 appears to be the most popular and best documented. The identification of the enterochromaffin substance as 5-hydroxytryptamine, however, contrasts with the fact that, by using several histochemical methods, no indole reactions have been obtained on formalin-fixed enterochromaffin granules6. We have reconsidered the problem by fixing duodenum, stomach and pancreas of various species, including guinea-pig, rat, mouse, rabbit, horse, pig, monkey and man, with 4 per cent formaldehyde or with 6 per cent glutaraldehyde in 0.1 molar phosphate buffer, pH 7.2, for 24 h.

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Affiliations

  1. Department of Pathological Anatomy, University of Pavia, Italy

    • E. SOLCIA
    •  & R. SAMPIETRO

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https://doi.org/10.1038/214196a0

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