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Effect of Human Growth Hormone on Methods of Insulin Immunoassay

Naturevolume 214pages195196 (1967) | Download Citation



Young and George1 reported that the immunoassay of insulin, using an anion exchange resin method of separation2, was affected by human growth hormone (HGH). Addition of HGH caused a decrease in the proportion of insulin bound to antibody. When added to albumin, HGH had no such effect. From these results, they concluded that HGH increased the amount of free insulin in serum by displacing it from an insulin–basic protein complex. Wright, Welborn and Fraser3 were unable to duplicate these results using a double–antibody immunoassay system and suggested that the findings of Young and George were an artefact of their anion-exchange resin.

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  1. 1

    Young, J. D., and George, E. P., Nature, 207, 1199 (1965).

  2. 2

    Meade, R. C., and Klitgaard, H. M., J. Nuc. Med., 3, 407 (1962).

  3. 3

    Wright, A. D., Welborn, T. A., and Fraser, T. R., Nature, 210, 418 (1966).

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Author information


  1. Radioisotope Service, Wood Veterans Administration Center and Department of Internal Medicine, Marquette University School of Medicine, Milwaukee, Wisconsin

    • R. C. MEADE
    •  & T. J. KLEIST


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Further reading

  • Serum insulin in acromegaly

    • H. Liebermeister
    • , H. G. Solbach
    • , W. H. Schilling
    • , R. R�enauver
    • , H. Meissner
    • , D. Gr�neklee
    • , L. Herberg
    •  & H. Daweke

    Diabetologia (1968)


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