Letter | Published:

Tobacco Necrosis Virus and its Satellite Virus

Naturevolume 214page178 (1967) | Download Citation



IN a recent letter1 Grogan and Uyemoto reported that they were unable to obtain cultures of tobacco necrosis virus (TNV) free from satellite virus (SV) after repeated isolation of single lesions from mixed inocula, and they quote Kassanis and Nixon2 as having the same experience. I write to point out that the paper quoted gives evidence to the contrary. It even shows photographic evidence of a gel-diffusion test to prove that when TNV is inoculated alone SV is not produced. Indeed, the fact that the Rothamsted tobacco necrosis virus contained two viruses and not two components of one, as previously seemed likely3, became evident when a culture of the same tobacco necrosis virus was found free from the other component4, now recognized as satellite virus. The different strains of TNV used in all subsequent work5–7 were free from SV and remained so as long as the plants in which they were propagated were grown in uninfested soil.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Grogan, R. G., and Uyemoto, J. K., Nature, 213, 705 (1967).

  2. 2

    Kassanis, B., and Nixon, H. L., J. Gen. Microbiol., 25, 459 (1961).

  3. 3

    Bawden, F. C., and Pirie, N. W., J. Gen. Microbiol., 4, 464 (1950).

  4. 4

    Kassanis, B., Ann. Inst. Phytopath. Benaki, 6, 7 (1965).

  5. 5

    Kassanis, B., J. Gen. Microbiol., 27, 477 (1962).

  6. 6

    Babos, P., and Kassanis, B., J. Gen. Microbiol., 32, 135 (1963).

  7. 7

    Kassanis, B., and Macfarlane, I., Virology, 26, 603 (1965).

  8. 8

    Kassanis, B., Proc. Intern. Conf. Plant Viruses, Wageningen, 1965, 177 (1966).

Download references

Author information


  1. Rothamsted Experimental Station, Harpenden, Hertfordshire



  1. Search for B. KASSANIS in:

About this article

Publication history


Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.