Abstract
IN a recent letter1 Grogan and Uyemoto reported that they were unable to obtain cultures of tobacco necrosis virus (TNV) free from satellite virus (SV) after repeated isolation of single lesions from mixed inocula, and they quote Kassanis and Nixon2 as having the same experience. I write to point out that the paper quoted gives evidence to the contrary. It even shows photographic evidence of a gel-diffusion test to prove that when TNV is inoculated alone SV is not produced. Indeed, the fact that the Rothamsted tobacco necrosis virus contained two viruses and not two components of one, as previously seemed likely3, became evident when a culture of the same tobacco necrosis virus was found free from the other component4, now recognized as satellite virus. The different strains of TNV used in all subsequent work5–7 were free from SV and remained so as long as the plants in which they were propagated were grown in uninfested soil.
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References
Grogan, R. G., and Uyemoto, J. K., Nature, 213, 705 (1967).
Kassanis, B., and Nixon, H. L., J. Gen. Microbiol., 25, 459 (1961).
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Kassanis, B., Ann. Inst. Phytopath. Benaki, 6, 7 (1965).
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Kassanis, B., and Macfarlane, I., Virology, 26, 603 (1965).
Kassanis, B., Proc. Intern. Conf. Plant Viruses, Wageningen, 1965, 177 (1966).
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KASSANIS, B. Tobacco Necrosis Virus and its Satellite Virus. Nature 214, 178 (1967). https://doi.org/10.1038/214178a0
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DOI: https://doi.org/10.1038/214178a0
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