Letter | Published:

Cyclic 3′,5′-Nucleotide Phosphodiesterase: Localization and Latent Activity in Rat Brain

Naturevolume 214pages9091 (1967) | Download Citation



THE pioneering investigations of Sutherland and his co-workers have focused considerable attention on adenosine 3′,5′-phosphate (cyclic AMP) and its relation to cellular metabolism. Cyclic AMP is synthesized by adenyl cyclase1 and destroyed by cyclic 3′,5′-nucleotide phosphodiesterase2. Brain cortex is of special interest in that it exhibits the highest activity in both cyclase3 and phosphodiesterase activities4, with phosphodiesterase present in overwhelming excess. We have evidence suggesting that phosphodiesterase in vivo might exist in a greatly inhibited state.5 Using the subcellular fractionation technique of De Robertis et al.6,7, we find that the enzyme is partly soluble and partly particulate, with a considerable portion concentrated inside nerve endings, and that ‘Triton X–100’ revealed substantial latent activity in a microsomal fraction.

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  1. LEON SALGANICOFF: Fellow of the Consejo Nacional de Investigaciones Cientificas y Tecnicas Republica Argentina.


  1. Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania



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