Letter | Published:

Effects of Puromycin and Actinomycin D on the Increase of Cystathionase and Cysteine Sulphinic Acid Decarboxylase Activities in the Liver of Thyroidectomized Rat

Naturevolume 214pages8890 (1967) | Download Citation



PREVIOUS investigations carried out in this laboratory have shown that the concentration of two cytoplasmic rat liver enzymes dependent on pyridoxal phosphate, namely, cysteine sulphinic acid (CSA) decarboxylase (L. cysteine sulphinate carboxylase E.C. and cystathionase (L. homoserine hydrolyase E.C., are adaptively increased in thyroidectomized animals1,2. The events underlying these changes in response to thyroidectomy are not known; however, we have suggested that the increase of activities may be attributable to an increased biosynthesis of the two apoenzymes3, and recently we have obtained results which agree with such a hypothesis4. The experiments reported here were undertaken in order to determine whether these increases are prevented by injections of inhibitors of protein biosynthesis—puromycin5—and of the DNA-directed RNA synthesis—actinomycin D (ref. 6). Both experiments were carried out in similar conditions except that in the first rats were given intraperitoneal injections of puromycin daily for 4 days at a dose each of 5 mg/100 g of body weight, whereas in the second actinomycin D was injected by the same route and for the same time at a dose of 3 µg/100 g of body weight. The animals were killed 4 h after the last injection. The amounts of inhibitor injected were chosen taking into consideration the report of Weber et al.7 and the results of preliminary assays.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Bergeret, B., Labouesse, J., and Chatagner, F., Bull. Soc. Chim. Biol., 40, 1923 (1958).

  2. 2

    Chatagner, F., Jollès-Bergeret, B., and Trautmann, O., Biochim. Biophys. Acta, 59, 744 (1962).

  3. 3

    Chatagner, F., and Jollès-Bergeret, B., in Chemical and Biological Aspects of Pyridoxal Catalysis (edit. by Snell, E. E., Fasella, P., Braunstein, A. E., and Rossi-Fanelli, A.), 477 (Pergamon Press, London, 1963).

  4. 4

    Chatagner, F., and Durieu-Trautmann, O., Nature, 207, 1390 (1965).

  5. 5

    Yarmonlinsky, M. B., and de la Haba, G. L., Proc. U.S. Nat. Acad. Sci., 45, 1721 (1959).

  6. 6

    Reich, E., Franklin, R. M., Shatkin, A. J., and Tatum, E. L., Science, 134, 556 (1961).

  7. 7

    Weber, G., Singhal, R. L., Stamm, N. B., Fisher, E. A., and Mentendiek, M. A., Adv. Enzyme Regulation, 2, 1 (1964).

  8. 8

    Chatagner, F., Jollès-Bergeret, B., and Labouesse, J., C.R. Acad. Sci., Paris, 251, 3097 (1960).

  9. 9

    Jollès-Bergeret, B., Labouesse, J., and Chatagner, F., Bull. Soc. Chim. Biol., 42, 51 (1960).

  10. 10

    Garren, L. D., Howell, R. R., Tomkins, G. M., and Crocco, R. M., Proc. U.S. Nat. Acad. Sci., 52, 1121 (1964).

  11. 11

    Rosen, F., Raina, P. N., Milholland, R. J., and Nichol, C. A., Science, 146, 661 (1964).

  12. 12

    Chatagner, F., and Rain, M. C., C.R. Acad. Sci., Paris, 261, 581 (1965).

  13. 13

    Durieu-Trautmann, O., and Chatagner, F., Bull. Soc. Chim. Biol., 48, 77 (1966).

  14. 14

    Knox, W. E., Adv. Enzyme Regulation, 2, 311 (1964).

  15. 15

    Schimke, R. T., Sweeney, E. W., and Berlin, C. M., J. Biol. Chem., 240, 322 (1965).

Download references

Author information


  1. Laboratoire de Chimie Biologique, 96, Boulevard Raspail, Paris, 6e



  1. Search for FERNANDE CHATAGNER in:

  2. Search for ODILE DURIEU-TRAUTMANN in:

  3. Search for MARIE-CHRISTINE RAIN in:

About this article

Publication history

Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.