Abstract
COLLAGEN synthesis, the principal differentiated function of the fibroblast, is carried out by homogeneous cell populations cultured under defined conditions1–5. Because hydroxy-proline is formed from proline in collagen, the rate of collagen synthesis can be determined with great sensitivity by measuring the incorporation of radioactive amino-acids3,6. This communication shows that different types of cell vary over a range of at least 104 in the rate at which they synthesize this protein. In cells of fibroblastic origin the property is, of course, most highly developed. In some non-fibroblastic cell types synthesis occurs at about 2–3 per cent of the rate characteristic of the fibroblast, while in others it is completely suppressed. The data indicate three possible levels at which the synthesis may be maintained in different cell types.
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GREEN, H., GOLDBERG, B. & TODARO, G. Differentiated Cell Types and the Regulation of Collagen Synthesis. Nature 212, 631–633 (1966). https://doi.org/10.1038/212631b0
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DOI: https://doi.org/10.1038/212631b0
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