Letter | Published:

Synthetic Approaches to the Biological Activity of β-Lactoglobulin in Human Allergy to Cows' Milk

Naturevolume 212pages541543 (1966) | Download Citation



WORK carried out in this laboratory since 1962 on the structure of the active sites in the allergens involved in the hypersensitivity syndrome of atopy in man (asthma, hay fever, atopic dermatitis group) has been based on the observation that the physicochemical properties of these allergens differ significantly from those of other (glyco)-protein antigens1. Specifically, the early postulate of N-glycosidic structural centres formed by Maillard reactions between ɛ-amino groups of lysine residues and reducing sugar has recently been substantiated by studying the absorption characteristics in the ultra-violet of purified inhalant and food allergens in relation to those of synthetic model compounds2,3. Because isolation studies on the atopic allergen in cows' milk had shown the skin-reactive allergen to reside largely in the β-lactoglobulin fraction4, an attempt was made to increase its specific activity by synthetically incorporating N-glycosidically bound carbohydrates into the molecule.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Berrens, L., Allergie Asthma, 8, 75 (1962).

  2. 2

    Berrens, L., and Bleumink, E., Intern. Arch. Allergy, 28, 150 (1965).

  3. 3

    Berrens, L., and Bleumink, E., Rec. Trav. Chim., 85, 59 (1966).

  4. 4

    Bleumink, E., Berrens, L., and Young, E., Sixth European Congr. Allergology, September, 1965, Stockholm.

  5. 5

    Aschaffenburg, R., and Drewry, J., Biochem. J., 65, 273 (1957).

  6. 6

    Berrens, L., Rec. Trav. Chim., 84, 1555 (1965).

  7. 7

    Keeney, M., and Bassette, E., J. Dairy Sci., 42, 445 (1959).

  8. 8

    Carpenter, K. J., Biochem. J., 77, 604 (1960).

  9. 9

    Berrens, L., and Young, E., Intern. Arch. Allergy, 19, 341 (1961).

Download references

Author information


  1. Department of Dermatology and Allergy, Academic Hosiptal, State University, Utrecht, The Netherlands

    •  & L. BERRENS


  1. Search for E. BLEUMINK in:

  2. Search for L. BERRENS in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.