Letter | Published:

Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken Pieces

Naturevolume 212pages534535 (1966) | Download Citation



DNA is presumably the primary site at which radiation damage has biological consequences1. Although lesions in single strands can be repaired after ultra-violet exposure2,3, it has not been shown conclusively that X-ray damage to bacterial DNA can be repaired. In order to study the latter problem, we have devised a method for assaying long pieces of single stranded DNA in order to facilitate the detection of a small number of breaks in single strands induced by X-rays.

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    Haynes, R. H., in Physical Processes in Radiation Biology, 51, edit. by Augenstein L., Mason, R., and Rosenberg, B. (Academic Press, New York, 1964).

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  1. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    • R. A. MCGRATH
    •  & R. W. WILLIAMS


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