Letter | Published:

Fluorescent Lipid from Brain

Naturevolume 212page510 (1966) | Download Citation



A SUBSTANCE has been obtained from the brain of rabbits killed with diisopropyl fluorophosphate that appears to have interesting properties and chemical structure. The substance can be obtained in amounts of 1.0–1.3 mg/g of brain by extraction with cold aqueous 5 per cent perchloric acid, followed by precipitation with phosphotungstic acid, and chromatography of the portion of the precipitate soluble in chloroform on silica with chloroform–methanol mixtures as eluant. The substance, obtained as a wax, gave a single fluorescent spot of RF 0.95 by thin layer chromatography on alumina with a pyridine–amyl alcohol–water (3 : 3 : 2) solvent system. The same areas also gave positive reactions with acidified permanganate, diazotized p-nitroaniline, Folin's phenol reagent, Schiff's fuchsin reagent, 2,4-dinitrophenylhydrazine, and with alkaline hydroxylamine followed by acidified ferric chloride solution. Elementary analysis showed some variation from lot to lot, with an average of 75.5 per cent carbon and 12.0 per cent hydrogen, and a range of ± 1 per cent for carbon and ±0.5 per cent for hydrogen.

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  1. Arthur P. Noyes Research Foundation, Norristown State Hospital, Norristown, Pennsylvania



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