Letter | Published:

Haemoglobin Association and the Sigmoid Oxygen Equilibrium Curve

Naturevolume 212page506 (1966) | Download Citation



REDUCED haemoglobin is generally considered to have the same molecular weight as oxyhaemoglobin. The osmotic pressure measurements of Adair1 and the light scattering experiments of Rossi-Fanelli, Antonini and Caputo2 agree with this concept, but our light scattering measurements have shown that reduced horse haemoglobin is in a state of close association when in 0.1 molar phosphate (pH 7.4).

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Adair, G. S., Proc. Roy. Soc., A, 120, 573 (1928).

  2. 2

    Rossi-Fanelli, A., Antonini, E., and Caputo, A., J. Biol. Chem., 236, 391 (1961).

  3. 3

    Hill, A. V., J. Physiol., 40, Proc. IV (1910).

  4. 4

    Douglas, C. G., Haldane, J. S., and Haldane, J. B. S., J. Physiol., 44, 275 (1912).

  5. 5

    Briehl, R. W., J. Biol. Chem., 238, 2361 (1963).

  6. 6

    Roughton, F. J. W., Biochem. J., 95, 58 (1965).

  7. 7

    Otis, A. B., and Roughton, F. J. W., Proc. Roy. Soc., B, 144, 55 (1955).

Download references

Author information


  1. Laboratory of Biochemistry I, University of Louvain, Belgium

    • P. PUTZEYS
    •  & H. REIJNAERS


  1. Search for P. PUTZEYS in:

  2. Search for H. REIJNAERS in:

About this article

Publication history

Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.