Letter | Published:

Method for the Preparation of Autoradiographs with Coverslip Cultures

Nature volume 212, pages 424425 (22 October 1966) | Download Citation

Subjects

Abstract

AUTORADIOGRAPHIC procedures are being increasingly used in all branches of cell biology. The routine methods are extremely useful with sections and air dried cell suspensions1–4, but the extension of these methods to coverslip cultures has often been unsatisfactory. The usual procedure for coverslip preparations involves cementing the coverslip cell side up on a slide in a drop of mounting medium (usually ‘Permount’) before covering with liquid emulsion or AR 10 stripping film5. This method often causes opaque emulsion patches in the space between coverslip and slide and the coverslip quite frequently floats off during rehydration. This communication describes a method which eliminates some of the problems encountered in autoradiography on coverslip cultures.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , Lab. Invest., 8, 71 (1959).

  2. 2.

    , Lab. Invest., 8, 131 (1959).

  3. 3.

    , and , J. Histochem. Cytochem., 10, 269 (1962).

  4. 4.

    , and , Exp. Cell. Res., 29, 430 (1962).

  5. 5.

    , and , Nature, 188, 511 (1960).

  6. 6.

    , and , Stain Technol., 33, 73 (1958).

  7. 7.

    , , and , Exp. Cell Res., 30, 229 (1963).

Download references

Author information

Author notes

    • V. R. BASRUR

    Present address: Princess Margaret's Hospital, Toronto, Canada.

Affiliations

  1. Division of Microscopic Anatomy, Ontario Veterinary College, Guelph, and Ontario Cancer Treatment and Research Foundation, Hamilton, Canada.

    • PARVATHI K. BASRUR
    • , V. R. BASRUR
    •  & J. P. W. GILMAN

Authors

  1. Search for PARVATHI K. BASRUR in:

  2. Search for V. R. BASRUR in:

  3. Search for J. P. W. GILMAN in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/212424a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.