Letter | Published:

Method for the Preparation of Autoradiographs with Coverslip Cultures

Naturevolume 212pages424425 (1966) | Download Citation



AUTORADIOGRAPHIC procedures are being increasingly used in all branches of cell biology. The routine methods are extremely useful with sections and air dried cell suspensions1–4, but the extension of these methods to coverslip cultures has often been unsatisfactory. The usual procedure for coverslip preparations involves cementing the coverslip cell side up on a slide in a drop of mounting medium (usually ‘Permount’) before covering with liquid emulsion or AR 10 stripping film5. This method often causes opaque emulsion patches in the space between coverslip and slide and the coverslip quite frequently floats off during rehydration. This communication describes a method which eliminates some of the problems encountered in autoradiography on coverslip cultures.

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    Herz, R. H., Lab. Invest., 8, 71 (1959).

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    Joftes, D. L., Lab. Invest., 8, 131 (1959).

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    Kopriwa, B. M., and Leblond, C. P., J. Histochem. Cytochem., 10, 269 (1962).

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    Prescott, D. M., and Bender, M. A., Exp. Cell. Res., 29, 430 (1962).

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    Krause, M., and Plaut, W., Nature, 188, 511 (1960).

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    Rothfels, K. H., and Siminovitch, L., Stain Technol., 33, 73 (1958).

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    Basrur, P. K., Basrur, V. R., and Gilman, J. P. W., Exp. Cell Res., 30, 229 (1963).

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    • V. R. BASRUR

    Present address: Princess Margaret's Hospital, Toronto, Canada


  1. Division of Microscopic Anatomy, Ontario Veterinary College, Guelph

    • , V. R. BASRUR
    •  & J. P. W. GILMAN
  2. Ontario Cancer Treatment and Research Foundation, Hamilton, Canada

    • , V. R. BASRUR
    •  & J. P. W. GILMAN


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