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Induction of Thymine Dimers in Synchronized Populations of Chinese Hamster Cells

An Erratum to this article was published on 07 January 1967

Abstract

RECENT evidence indicates that the response of Chinese hamster cells in vitro to ultra-violet radiation changes during the difference phases of the cell cycle1,2,4. Humphrey et al.1 have shown that cells synthesizing deoxyribo-nucleic acid (DNA) at the time of irradiation using ultraviolet rays (S phase cells) sustain more chromosome damage than cells which were in the period before DNA synthesis (G1 or cells in the period after DNA synthesis (G2). Sinclair and Morton have demonstrated that the response to ultra-violet radiation in synchronous populations of cells as measured by cell death also fluctuates with the age of the irradiated cells; the fraction of surviving cells falls as the cells move from G1 into S phase, and rises as they move from S into G2 phase2. In an effort to explain these changes in radiation response during the cell cycle, the production of an ultra-violet photoproduct of DNA, the thymine dimer3 has been measured in synchronized population of Chinese hamster cells4.

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References

  1. Humphrey, R. M., Dewey, W. C., and Cork, A., Rad. Res., 19, 247 (1963).

    Article  CAS  Google Scholar 

  2. Sinclair, W. K., and Morton, R. A., Biophys. J., 5, 1 (1965).

    Article  CAS  Google Scholar 

  3. Beukers, R., and Berends, W., Biochim. Biophys. Acta, 41, 550 (1960).

    Article  CAS  Google Scholar 

  4. Trosko, J. E., Kassachau, M., Covington, L., and Chu, E. H. Y., Rad. Res., 27, 535 (1966).

    Google Scholar 

  5. Hsu, T. C., Dewey, W. C., and Humphrey, R. M., Exp. Cell Res., 27, 441 (1962).

    Article  CAS  Google Scholar 

  6. Stubblefield, E., and Klevecz, R., Exp. Cell Res., 40, 660 (1965).

    Article  CAS  Google Scholar 

  7. Terasima, T., and Tolmach, L. J., Exp. Cell Res., 30, 334 (1963).

    Article  Google Scholar 

  8. Setlow, R. B., Swenson, P. A., and Carrier, W. L., Science, 142, 1464 (1963).

    Article  ADS  CAS  Google Scholar 

  9. Setlow, R. B., and Carrier, W. L., Proc. U.S. Nat. Acad. Sci., 51, 226 (1964).

    Article  ADS  CAS  Google Scholar 

  10. Boyce, R., and Howard-Flanders, P., Proc. U.S. Nat. Acad. Sci., 51, 293 (1964).

    Article  ADS  CAS  Google Scholar 

  11. Trosko, J. E., Chu, E. H. Y., and Carrier, W. L., Rad. Res., 24, 667 (1965).

    Article  CAS  Google Scholar 

  12. Humphrey, R. M., Dewey, W. C., and Steward, D. L., Third Intern. Cong. Rad. Res. (abstracts) 1966.

  13. Johns, H. E., Rapaport, S. A. A., and Delbruch, M., J. Mol. Biol., 4, 104 (1962).

    Article  CAS  Google Scholar 

  14. Setlow, R. B., and Setlow, J. K., Proc. U.S. Nat. Acad. Sci., 42, 1250 (1962).

    Article  ADS  Google Scholar 

  15. Setlow, R. B., Biochim. Biophys. Acta, 49, 237 (1961).

    Article  CAS  Google Scholar 

  16. Wacker, A., Dellweg, H., and Jacherts, D., J. Mol. Biol., 4, 413 (1962).

    Article  CAS  Google Scholar 

  17. Swenson, P. A., and Setlow, R. B., J. Mol. Biol., 15, 201 (1966).

    Article  CAS  Google Scholar 

  18. Chu, E. H. Y., Mutation Res., 2, 75 (1965).

    Article  CAS  Google Scholar 

Download references

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STEWARD, D., HUMPHREY, R. Induction of Thymine Dimers in Synchronized Populations of Chinese Hamster Cells. Nature 212, 298–300 (1966). https://doi.org/10.1038/212298b0

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